Chagas Disease and Leishmaniasis, Sympatric Zoonoses Present in Human from Rural Communities of Venezuela

• Published in: Acta parasitologica 2024-03, Vol.69 (1), p.616-627
• Main Authors: Ferrer, Elizabeth, Aguilar, Cruz M., Viettri, Mercedes, Torrellas, Annhymariet, Lares, María, Diaz, Marietta, Delgado, Olinda, Feliciangeli, María D., Herrera, Leidi
• Format: Article
• Language: English
• Subjects: Animal Systematics/Taxonomy/Biogeography; Biomedical and Life Sciences; Biomedicine; Blood; Blood clots; Blood coagulation; Chagas disease; Control programs; Ecology; Enzyme-linked immunosorbent assay; Hsp60 protein; Immunofluorescence; Indirect hemagglutination; Leishmania; Leishmaniasis; Leishmanin; Medical Microbiology; Microbiology; Original Paper; Parasitic diseases; Parasitology; Protozoa; Rural areas; Rural communities; Serology; Skin tests; Sympatric populations; Tropical diseases; Trypanosoma cruzi; Vector-borne diseases
• ISSN: 1230-2821; 1896-1851; 1896-1851
• DOI: 10.1007/s11686-023-00786-8

Purpose Trypanosoma cruzi and Leishmania spp. coexist in several endemic areas, and there are few studies of Chagas disease and leishmaniasis coinfection worldwide; for this reason, the objective of this work was to determine the Chagas disease and leishmaniasis coinfection in several rural communities co-endemic for these diseases. Methods A total of 1107 human samples from six co-endemic rural communities of Cojedes state, Venezuela, were analyzed. Serum samples were evaluated by ELISA, indirect hemagglutination, and indirect immunofluorescence for Chagas disease diagnosis, and individuals were evaluated for leishmaniasis by leishmanin skin test (LST). Approximately, 30% of the individuals were also analyzed by PCR (blood clot samples) for T. cruzi and for Leishmania spp. Results The 14.7% of the individuals were positive to Trypanosoma cruzi infection by serology, and 25.8% were positive to Leishmania spp. current or past infection by LST. Among the group with PCR results, 7.8% were positive for T. cruzi , and 9.4% for Leishmania spp. The coinfection T. cruzi / Leishmania spp. was 6.5%. The T. cruzi DTUs of the positive blood clot samples were TcI, revealed using the molecular markers: (i) intergenic region of the miniexon, (ii) D7 divergent domain of the 24Sα rDNA, (iii) size-variable domain of the 18S rDNA, and (iv) hsp60-PCR–RFLP (EcoRV). The Leishmania species identified were L. (Leishmania) mexicana and L. (Viannia) braziliensis. Conclusion A high prevalence was found for T. cruzi and Leishmania spp. single and coinfections in almost all communities studied, being these results of relevance for the implementation of control programs in co-endemic areas.