Loading…

Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways

UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metall...

Full description

Saved in:
Bibliographic Details
Published in:Photochemical & photobiological sciences 2024-03, Vol.23 (3), p.463-478
Main Authors: Liu, Meiling, Huang, Shaokai, Park, Sunmin
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423
container_end_page 478
container_issue 3
container_start_page 463
container_title Photochemical & photobiological sciences
container_volume 23
creator Liu, Meiling
Huang, Shaokai
Park, Sunmin
description UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm 2 for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha ( TNF-α ), and inducible nitric oxide synthase ( iNOS ) expression, while they concurrently enhanced collagen-1 ( COL1A1 ), β-catenin ( CTNNB1 ), and superoxide dismutase type-1 ( SOD1 ) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in MMP1 expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting MMP1 expression and elevating β-catenin expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.
doi_str_mv 10.1007/s43630-023-00531-0
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2923909359</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2923909359</sourcerecordid><originalsourceid>FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423</originalsourceid><addsrcrecordid>eNp9kbtOHTEYhK0oCMgJL5Aicplmg-1_b6YjKBckEClCks7y-nKOya692F7QeYU8dQwHKFPZmn_mK2YQekfJR0pId5xqaIFUhEFFSAO0Iq_QIa27uuKEs9cv_-b3AXqT0g0htKnbbh8dQA-sbTkcor_nfuMGl0PcYmOtUTnhYPHgglTZ3RmswjSHxesie3z981PlvF6U0XjehBy0nOTaYOfxZpmkx39MlNn5oLbZpBM8Bb2MRSjRAr28_E6x9Br_8hknt_ZydH6NZ5k393Kb3qI9K8dkjp7eFbr-8vnH2bfq4urr-dnpRaUY73OlJbChZpZCB6B5V9Oup21nG14EOwAQToaubxU31FiqWlJylvcWCGtMzWCFPuy4cwy3i0lZTC4pM47Sm7AkwTiDUiAU4AqxnVXFkFI0VszRTTJuBSXiYQOx20CUDcTjBoKU0Psn_jJMRr9EnksvBtgZUjn5tYniJiyxtJH-h_0HJjKTGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2923909359</pqid></control><display><type>article</type><title>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</title><source>Springer Link</source><creator>Liu, Meiling ; Huang, Shaokai ; Park, Sunmin</creator><creatorcontrib>Liu, Meiling ; Huang, Shaokai ; Park, Sunmin</creatorcontrib><description>UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm 2 for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha ( TNF-α ), and inducible nitric oxide synthase ( iNOS ) expression, while they concurrently enhanced collagen-1 ( COL1A1 ), β-catenin ( CTNNB1 ), and superoxide dismutase type-1 ( SOD1 ) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in MMP1 expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting MMP1 expression and elevating β-catenin expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</description><identifier>ISSN: 1474-905X</identifier><identifier>EISSN: 1474-9092</identifier><identifier>DOI: 10.1007/s43630-023-00531-0</identifier><identifier>PMID: 38326693</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>beta Catenin - metabolism ; beta Catenin - pharmacology ; Biochemistry ; Biomaterials ; Cell Line ; Chemistry ; Chemistry and Materials Science ; Collagen - pharmacology ; Humans ; Keratinocytes - metabolism ; Matrix Metalloproteinase 1 - metabolism ; Matrix Metalloproteinase 1 - pharmacology ; Original Papers ; Physical Chemistry ; Plant Sciences ; Reactive Oxygen Species - metabolism ; Skin Aging ; Ultraviolet Rays ; Wnt Signaling Pathway</subject><ispartof>Photochemical &amp; photobiological sciences, 2024-03, Vol.23 (3), p.463-478</ispartof><rights>The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423</cites><orcidid>0000-0002-6092-8340</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38326693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Meiling</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><title>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</title><title>Photochemical &amp; photobiological sciences</title><addtitle>Photochem Photobiol Sci</addtitle><addtitle>Photochem Photobiol Sci</addtitle><description>UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm 2 for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha ( TNF-α ), and inducible nitric oxide synthase ( iNOS ) expression, while they concurrently enhanced collagen-1 ( COL1A1 ), β-catenin ( CTNNB1 ), and superoxide dismutase type-1 ( SOD1 ) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in MMP1 expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting MMP1 expression and elevating β-catenin expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</description><subject>beta Catenin - metabolism</subject><subject>beta Catenin - pharmacology</subject><subject>Biochemistry</subject><subject>Biomaterials</subject><subject>Cell Line</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Collagen - pharmacology</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Matrix Metalloproteinase 1 - pharmacology</subject><subject>Original Papers</subject><subject>Physical Chemistry</subject><subject>Plant Sciences</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Skin Aging</subject><subject>Ultraviolet Rays</subject><subject>Wnt Signaling Pathway</subject><issn>1474-905X</issn><issn>1474-9092</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kbtOHTEYhK0oCMgJL5Aicplmg-1_b6YjKBckEClCks7y-nKOya692F7QeYU8dQwHKFPZmn_mK2YQekfJR0pId5xqaIFUhEFFSAO0Iq_QIa27uuKEs9cv_-b3AXqT0g0htKnbbh8dQA-sbTkcor_nfuMGl0PcYmOtUTnhYPHgglTZ3RmswjSHxesie3z981PlvF6U0XjehBy0nOTaYOfxZpmkx39MlNn5oLbZpBM8Bb2MRSjRAr28_E6x9Br_8hknt_ZydH6NZ5k393Kb3qI9K8dkjp7eFbr-8vnH2bfq4urr-dnpRaUY73OlJbChZpZCB6B5V9Oup21nG14EOwAQToaubxU31FiqWlJylvcWCGtMzWCFPuy4cwy3i0lZTC4pM47Sm7AkwTiDUiAU4AqxnVXFkFI0VszRTTJuBSXiYQOx20CUDcTjBoKU0Psn_jJMRr9EnksvBtgZUjn5tYniJiyxtJH-h_0HJjKTGg</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Liu, Meiling</creator><creator>Huang, Shaokai</creator><creator>Park, Sunmin</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></search><sort><creationdate>20240301</creationdate><title>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</title><author>Liu, Meiling ; Huang, Shaokai ; Park, Sunmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>beta Catenin - metabolism</topic><topic>beta Catenin - pharmacology</topic><topic>Biochemistry</topic><topic>Biomaterials</topic><topic>Cell Line</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Collagen - pharmacology</topic><topic>Humans</topic><topic>Keratinocytes - metabolism</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Matrix Metalloproteinase 1 - pharmacology</topic><topic>Original Papers</topic><topic>Physical Chemistry</topic><topic>Plant Sciences</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Skin Aging</topic><topic>Ultraviolet Rays</topic><topic>Wnt Signaling Pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Meiling</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemical &amp; photobiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Meiling</au><au>Huang, Shaokai</au><au>Park, Sunmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</atitle><jtitle>Photochemical &amp; photobiological sciences</jtitle><stitle>Photochem Photobiol Sci</stitle><addtitle>Photochem Photobiol Sci</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>23</volume><issue>3</issue><spage>463</spage><epage>478</epage><pages>463-478</pages><issn>1474-905X</issn><eissn>1474-9092</eissn><abstract>UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm 2 for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha ( TNF-α ), and inducible nitric oxide synthase ( iNOS ) expression, while they concurrently enhanced collagen-1 ( COL1A1 ), β-catenin ( CTNNB1 ), and superoxide dismutase type-1 ( SOD1 ) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in MMP1 expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting MMP1 expression and elevating β-catenin expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38326693</pmid><doi>10.1007/s43630-023-00531-0</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 1474-905X
ispartof Photochemical & photobiological sciences, 2024-03, Vol.23 (3), p.463-478
issn 1474-905X
1474-9092
language eng
recordid cdi_proquest_miscellaneous_2923909359
source Springer Link
subjects beta Catenin - metabolism
beta Catenin - pharmacology
Biochemistry
Biomaterials
Cell Line
Chemistry
Chemistry and Materials Science
Collagen - pharmacology
Humans
Keratinocytes - metabolism
Matrix Metalloproteinase 1 - metabolism
Matrix Metalloproteinase 1 - pharmacology
Original Papers
Physical Chemistry
Plant Sciences
Reactive Oxygen Species - metabolism
Skin Aging
Ultraviolet Rays
Wnt Signaling Pathway
title Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T08%3A13%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effects%20of%20bioactive%20compounds%20on%20UVB-induced%20photodamage%20in%20human%20keratinocytes:%20modulation%20of%20MMP1%20and%20Wnt%20signaling%20pathways&rft.jtitle=Photochemical%20&%20photobiological%20sciences&rft.au=Liu,%20Meiling&rft.date=2024-03-01&rft.volume=23&rft.issue=3&rft.spage=463&rft.epage=478&rft.pages=463-478&rft.issn=1474-905X&rft.eissn=1474-9092&rft_id=info:doi/10.1007/s43630-023-00531-0&rft_dat=%3Cproquest_cross%3E2923909359%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2923909359&rft_id=info:pmid/38326693&rfr_iscdi=true