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Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways
UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metall...
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Published in: | Photochemical & photobiological sciences 2024-03, Vol.23 (3), p.463-478 |
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description | UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm
2
for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha (
TNF-α
), and inducible nitric oxide synthase (
iNOS
) expression, while they concurrently enhanced collagen-1 (
COL1A1
), β-catenin (
CTNNB1
), and superoxide dismutase type-1 (
SOD1
) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in
MMP1
expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting
MMP1
expression and elevating
β-catenin
expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health. |
doi_str_mv | 10.1007/s43630-023-00531-0 |
format | article |
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2
for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha (
TNF-α
), and inducible nitric oxide synthase (
iNOS
) expression, while they concurrently enhanced collagen-1 (
COL1A1
), β-catenin (
CTNNB1
), and superoxide dismutase type-1 (
SOD1
) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in
MMP1
expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting
MMP1
expression and elevating
β-catenin
expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</description><identifier>ISSN: 1474-905X</identifier><identifier>EISSN: 1474-9092</identifier><identifier>DOI: 10.1007/s43630-023-00531-0</identifier><identifier>PMID: 38326693</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>beta Catenin - metabolism ; beta Catenin - pharmacology ; Biochemistry ; Biomaterials ; Cell Line ; Chemistry ; Chemistry and Materials Science ; Collagen - pharmacology ; Humans ; Keratinocytes - metabolism ; Matrix Metalloproteinase 1 - metabolism ; Matrix Metalloproteinase 1 - pharmacology ; Original Papers ; Physical Chemistry ; Plant Sciences ; Reactive Oxygen Species - metabolism ; Skin Aging ; Ultraviolet Rays ; Wnt Signaling Pathway</subject><ispartof>Photochemical & photobiological sciences, 2024-03, Vol.23 (3), p.463-478</ispartof><rights>The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423</cites><orcidid>0000-0002-6092-8340</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38326693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Meiling</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><title>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</title><title>Photochemical & photobiological sciences</title><addtitle>Photochem Photobiol Sci</addtitle><addtitle>Photochem Photobiol Sci</addtitle><description>UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm
2
for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha (
TNF-α
), and inducible nitric oxide synthase (
iNOS
) expression, while they concurrently enhanced collagen-1 (
COL1A1
), β-catenin (
CTNNB1
), and superoxide dismutase type-1 (
SOD1
) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in
MMP1
expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting
MMP1
expression and elevating
β-catenin
expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</description><subject>beta Catenin - metabolism</subject><subject>beta Catenin - pharmacology</subject><subject>Biochemistry</subject><subject>Biomaterials</subject><subject>Cell Line</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Collagen - pharmacology</subject><subject>Humans</subject><subject>Keratinocytes - metabolism</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Matrix Metalloproteinase 1 - pharmacology</subject><subject>Original Papers</subject><subject>Physical Chemistry</subject><subject>Plant Sciences</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Skin Aging</subject><subject>Ultraviolet Rays</subject><subject>Wnt Signaling Pathway</subject><issn>1474-905X</issn><issn>1474-9092</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kbtOHTEYhK0oCMgJL5Aicplmg-1_b6YjKBckEClCks7y-nKOya692F7QeYU8dQwHKFPZmn_mK2YQekfJR0pId5xqaIFUhEFFSAO0Iq_QIa27uuKEs9cv_-b3AXqT0g0htKnbbh8dQA-sbTkcor_nfuMGl0PcYmOtUTnhYPHgglTZ3RmswjSHxesie3z981PlvF6U0XjehBy0nOTaYOfxZpmkx39MlNn5oLbZpBM8Bb2MRSjRAr28_E6x9Br_8hknt_ZydH6NZ5k393Kb3qI9K8dkjp7eFbr-8vnH2bfq4urr-dnpRaUY73OlJbChZpZCB6B5V9Oup21nG14EOwAQToaubxU31FiqWlJylvcWCGtMzWCFPuy4cwy3i0lZTC4pM47Sm7AkwTiDUiAU4AqxnVXFkFI0VszRTTJuBSXiYQOx20CUDcTjBoKU0Psn_jJMRr9EnksvBtgZUjn5tYniJiyxtJH-h_0HJjKTGg</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Liu, Meiling</creator><creator>Huang, Shaokai</creator><creator>Park, Sunmin</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></search><sort><creationdate>20240301</creationdate><title>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</title><author>Liu, Meiling ; Huang, Shaokai ; Park, Sunmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c298t-da32b42f13733d974178167f59373fb33090b786c9e1ef1c60c29f98f3025e423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>beta Catenin - metabolism</topic><topic>beta Catenin - pharmacology</topic><topic>Biochemistry</topic><topic>Biomaterials</topic><topic>Cell Line</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Collagen - pharmacology</topic><topic>Humans</topic><topic>Keratinocytes - metabolism</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Matrix Metalloproteinase 1 - pharmacology</topic><topic>Original Papers</topic><topic>Physical Chemistry</topic><topic>Plant Sciences</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Skin Aging</topic><topic>Ultraviolet Rays</topic><topic>Wnt Signaling Pathway</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Meiling</creatorcontrib><creatorcontrib>Huang, Shaokai</creatorcontrib><creatorcontrib>Park, Sunmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemical & photobiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Meiling</au><au>Huang, Shaokai</au><au>Park, Sunmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways</atitle><jtitle>Photochemical & photobiological sciences</jtitle><stitle>Photochem Photobiol Sci</stitle><addtitle>Photochem Photobiol Sci</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>23</volume><issue>3</issue><spage>463</spage><epage>478</epage><pages>463-478</pages><issn>1474-905X</issn><eissn>1474-9092</eissn><abstract>UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm
2
for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha (
TNF-α
), and inducible nitric oxide synthase (
iNOS
) expression, while they concurrently enhanced collagen-1 (
COL1A1
), β-catenin (
CTNNB1
), and superoxide dismutase type-1 (
SOD1
) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in
MMP1
expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting
MMP1
expression and elevating
β-catenin
expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>38326693</pmid><doi>10.1007/s43630-023-00531-0</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-6092-8340</orcidid></addata></record> |
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subjects | beta Catenin - metabolism beta Catenin - pharmacology Biochemistry Biomaterials Cell Line Chemistry Chemistry and Materials Science Collagen - pharmacology Humans Keratinocytes - metabolism Matrix Metalloproteinase 1 - metabolism Matrix Metalloproteinase 1 - pharmacology Original Papers Physical Chemistry Plant Sciences Reactive Oxygen Species - metabolism Skin Aging Ultraviolet Rays Wnt Signaling Pathway |
title | Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways |
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