Impact of the Different Chemical-Based Decellularization Protocols on the Properties of the Caprine Pericardium

Purpose This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations. Methods Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C compr...

Full description

Saved in:
Bibliographic Details
Published in:Cardiovascular engineering and technology 2024-06, Vol.15 (3), p.279-289
Main Authors: Deepak, Thirumalai, Bajhaiya, Deepak, Babu, Anju R.
Format: Article
Language:English
Subjects:
Animals
Biomechanical Phenomena
Biomechanics
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Biomedicine
Cardiology
Decellularized Extracellular Matrix - chemistry
Decellularized Extracellular Matrix - pharmacology
Detergents
DNA
Elastic Modulus
Goats
Octoxynol - chemistry
Octoxynol - pharmacology
Original Article
Pericardium
Pericardium - cytology
Pericardium - drug effects
Protocol
Sodium dodecyl sulfate
Sodium Dodecyl Sulfate - pharmacology
Surface-Active Agents - chemistry
Surface-Active Agents - pharmacology
Surfactants
Tensile stress
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations. Methods Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100. Results Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus ( p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue. Conclusion The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations. Graphical Abstract
ISSN:1869-408X
1869-4098
1869-4098
DOI:10.1007/s13239-024-00712-7