Preparation and synbiotic interaction mechanism of microcapsules of Bifidobacterium animalis F1–7 and human milk oligosaccharides (HMO)

• Published in: International journal of biological macromolecules 2024-02, Vol.259 (Pt 1), p.129152-129152, Article 129152
• Main Authors: Huang, Xiaoyang, Liu, Rui, Wang, Jing, Bao, Yuexin, Yi, Huaxi, Wang, Xiaohong, Lu, Youyou
• Format: Article
• Language: English
• Subjects: 6′-Sialyllactose; Bifidobacterium animalis subsp. lactis F1–7; Emulsification/internal gelation; Human milk oligosaccharides; Intestinal flora; Microcapsules
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.129152

Probiotics such as Bifidobacterium spp. generally possess important physiological functions. However, maintaining probiotic viability is a challenge during processing, storage, and digestive transit period. Microencapsulation is widely considered to be an attractive approach. In this study, B. animalis F1–7 microcapsules and B. animalis F1–7-HMO microcapsules were successfully prepared by emulsification/internal gelation with high encapsulation efficiency (90.67 % and 92.16 %, respectively). The current study revealed that HMO-supplemented microcapsules exhibited more stable lyophilized forms and thermal stability. Additionally, a significant improvement in probiotic cell viability was observed in such microcapsules during simulated gastrointestinal (GI) fluids or storage. We also showed that the individual HMO mixtures 6′-SL remarkably promoted the growth and acetate yield of B. animalis F1–7 for 48 h (p