Comparing tonic and phasic dendritic calcium in cholinergic pedunculopontine neurons and dopaminergic substantia nigra neurons

• Published in: The European journal of neuroscience 2024-04, Vol.59 (7), p.1638-1656
• Main Authors: Chen, Rita Yu‐Tzu, Evans, Rebekah C.
• Format: Article
• Language: English
• Subjects: Brain stem; Calcium channels; Calcium imaging; Calcium influx; Calcium signalling; Dopamine receptors; Electrophysiology; Firing rate; Movement disorders; Neurodegenerative diseases; Neurons; Nifedipine; Parkinson's disease; Pedunculopontine tegmental nucleus; Substantia nigra
• ISSN: 0953-816X; 1460-9568
• DOI: 10.1111/ejn.16281

Several brainstem nuclei degenerate in Parkinson's disease (PD). In addition to the well‐characterized dopaminergic neurons of the substantia nigra pars compacta (SNc), the cholinergic neurons of the pedunculopontine nucleus (PPN) also degenerate in PD. One leading hypothesis of selective vulnerability is that pacemaking activity and the activation of low‐threshold L‐type calcium current are major contributors to tonic calcium load and cellular stress in SNc dopaminergic neurons. However, it is not yet clear whether the vulnerable PPN cholinergic neurons share this property. Therefore, we used two‐photon dendritic calcium imaging and whole‐cell electrophysiology to evaluate the role of L‐type calcium channels in tonic and phasic dendritic calcium signals in PPN and SNc neurons. In addition, we investigated N‐ and P/Q‐type calcium channel regulation of firing properties and dendritic calcium in PPN neurons. We found that blocking L‐type channels reduces tonic firing rate and dendritic calcium levels in SNc neurons. By contrast, the tonic calcium load in PPN neurons did not depend on L‐, N‐ or P/Q‐type channels. However, we found that blocking either L‐type (with nifedipine) or N‐ and P/Q‐type (with omega‐conotoxin MVIIC) channels reduces phasic calcium influx in PPN dendrites. Together, these findings show that L‐type calcium channels play different roles in the activity of SNc and PPN neurons, and suggest that low‐threshold L‐type channels are not responsible for tonic calcium levels in PPN cholinergic neurons and are therefore not likely to be a source of selective vulnerability in these cells. Chen and Evans show that tonic and phasic calcium in the dendrites of neurons vulnerable to degeneration in Parkinson's disease are regulated by different calcium channels. L‐type and N‐ or P/Q‐type calcium channels support phasic, but not tonic, calcium influx into cholinergic pedunculopontine nucleus neurons. By contrast, dopaminergic neurons of the substantia nigra pars compacta rely on L‐type calcium channels for both tonic and phasic calcium.