Sequencing analysis and efficient biodiesel production by lipase from Pseudomonas aeruginosa

• Published in: Molecular biology reports 2024-12, Vol.51 (1), p.323-323, Article 323
• Main Authors: AL-Kadmy, Israa M. S., Aziz, Sarah Naji, Hussein, Nadheema Hammood, El-shafeiy, Samah N., Hamzah, Israa Hussein, Suhail, Ahmed, alhomaidi, Eman, Algammal, Abdelazeem M., El-Saber Batiha, Gaber, ElBadre, Hala M, Hetta, Helal F.
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Base Sequence; Biodiesel fuels; Biofuels; Biomedical and Life Sciences; Diesel; Gas chromatography; Histology; Humans; Life Sciences; Lipase; Lipase - metabolism; Morphology; Oils; Original Article; Plant Oils - chemistry; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Pseudomonas Infections; Sequence analysis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-023-09156-z

Background Recently, lipase processing for biodiesel production has shown a global increase as it is considered a potential alternative clean-fuel source. The current study’s objective is to investigate of lipolytic activity of lipase produced from different strains of Pseudomonas aeruginosa ( P. aeruginosa ) in biodiesel production using edible plant oils. The goal is to develop an efficient and cost-effective method for producing inexpensive and environmentally friendly biodiesel. Methods and results Four P. aeruginosa isolates were obtained from different environmental sources (soil), phenotypically identified, and it was confirmed by the PCR detection of the 16SrRNA gene. The isolated P. aeruginosa strains were screened for lipase production, and the recovered lipase was purified. Besides, the lipase ( lip ) gene was detected by PCR, and the purified PCR products were sequenced and analyzed. The production of biofuel was conducted using gas chromatography among tested oils. It was found that castor oil was the best one that enhances lipase production in-vitro .