Effect of different crosslinking agents on hybrid chitosan/collagen hydrogels for potential tissue engineering applications

Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this stu...

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Published in:International journal of biological macromolecules 2024-04, Vol.263 (Pt 2), p.129858-129858, Article 129858
Main Authors: Sánchez-Cid, Pablo, Alonso-González, María, Jiménez-Rosado, Mercedes, Benhnia, Mohammed Rafii-El-Idrissi, Ruiz-Mateos, E., Ostos, Francisco J., Romero, Alberto, Perez-Puyana, Víctor M.
Format: Article
Language:English
Subjects:
Biocompatibility
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biopolymers
Chemical crosslinking
Chitosan
Chitosan - chemistry
Collagen
Collagen - chemistry
Cross-Linking Reagents - chemistry
Hydrogels - chemistry
Hydrogels - pharmacology
Rheology
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
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Summary:Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this study, the microstructure and rheological performance, as well as functional and biological properties of chitosan and collagen hydrogels (CH/CG) crosslinked with different coupling agents, both natural such as d-Fructose (F), genipin (G) and transglutaminase (T) and synthetic, using a combination of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) will be assessed. FTIR tests were carried out to determine if the proposed crosslinking reactions for each crosslinking agent occurred as expected, obtaining positive results in this aspect. Regarding the characterization of the properties of each system, two main trends were observed, from which it could be established that crosslinking with G and EDC-NHS turned out to be more effective and beneficial than with the other two crosslinking agents, producing significant improvements with respect to the base CH/CG hydrogel. In addition, in vitro tests demonstrated the potential application in TE of these systems, especially for those crosslinked with G, T and EDC-NHS.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2024.129858