Optimization of bacterial cellulose production by Komagataeibacter sucrofermentans in synthetic media and agrifood side streams supplemented with organic acids and vitamins

• Published in: Bioresource technology 2024-04, Vol.398, p.130511-130511, Article 130511
• Main Authors: Adamopoulou, Vasiliki, Bekatorou, Argyro, Brinias, Vasilios, Michalopoulou, Panagiota, Dimopoulos, Charalampos, Zafeiropoulos, John, Petsi, Theano, Koutinas, Athanasios A.
• Format: Article
• Language: English
• Subjects: Antioxidant capacity; Drying method; Response Surface Methodology; Surface and texture analysis; Yield prediction and optimization
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2024.130511

[Display omitted] •Thiamine, ascorbic, citric, and gallic acid affect bacterial cellulose production.•Higher BC yield (19.4 g/L) obtained in the fortified raisins side stream extracts.•Yield in non-fortified raisin extract-orange juice-green tea substrate was 5.9 g/L.•Response Surface Methodology is reliable for BC yield prediction and optimization.•BC textural and antioxidant properties are affected by substrate and drying method. The effect of thiamine (TA), ascorbic acid (AA), citric acid, and gallic acid (GA) on bacterial cellulose (BC) production by Komagataeibacter sucrofermentans, in synthetic (Hestrin and Schramm, HS) and natural substrates (industrial raisins finishing side stream extract, FSSE; orange juice, OJ; green tea extract, GTE), was investigated. The Response Surface Methodology was found reliable for BC yield prediction and optimization. Higher yields were achieved in the FSSE substrates, especially those supplemented with AA, TA, and GA (up to 19.4 g BC/L). The yield in the non-fortified substrates was 1.1–5.4 and 11.6–15.7 g/L, in HS and FSSE, respectively. The best yield in the natural non-fortified substrate FSSE-OJ-GTE (50–20-30 %), was 5.9 g/L. The porosity, crystallinity, and antioxidant properties of the produced BC films were affected by both the substrate and the drying method (freeze- or oven-drying). The natural substrates and the process wastewaters can be further exploited towards added value and sustainability. Take Home Message Sentence: Raisin and citrus side-streams can be efficiently combined for bacterial cellulose production, enhanced by other vitamin- and phenolic-rich substrates such as green tea.