Molecular detection of ovine gammaherpesvirus 2 in free ranging wild boars (Sus scrofa) from Southern Brazil

• Published in: Brazilian journal of microbiology 2024-06, Vol.55 (2), p.1923-1929
• Main Authors: Headley, Selwyn Arlington, Yasumitsu, Carolina Yuka, Dall Agnol, Alais Maria, de Souza, Tatiana Carolina Gomes Dutra, da Silva Porto, Gisele, Leme, Raquel Arruda, Alfieri, Alice Fernandes, Alfieri, Amauri Alcindo
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Brazil; DNA, Viral - genetics; Food Microbiology; Gammaherpesvirinae - classification; Gammaherpesvirinae - genetics; Gammaherpesvirinae - isolation & purification; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Life Sciences; Lung - virology; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Phylogeny; Sus scrofa - virology; Swine; Swine Diseases - virology; Veterinary and Animal Production - Research Paper
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1007/s42770-024-01302-4

Ovine gammaherpesvirus 2 (OvGHV2) is a member of Macavirus genus, subfamily Gammaherpesvirinae , family Herpesviridae , and causes sheep associated-malignant catarrhal fever (SA-MCF) in a wide range of ungulates. However, no descriptions of SA-MCF and/or infections due to OvGHV2 were identified in the wild boar ( Sus scrofa ). This study investigated the occurrence of OvGHV2 in the lungs (n = 44) of asymptomatic, free ranging wild boars captured in several regions of Paraná State, Southern Brazil. A PCR assay targeting the OvGHV2 tegument protein gene amplified OvGHV2 DNA in 4.55% (2/44) of the pulmonary tissues evaluated. Sequence analysis confirmed that the OvGHV2 strains herein identified have 98.4% deduced amino acid (aa) sequence identity with the prototype strain of OvGHV2 and 96.4–100% aa identity with similar strains of OvGHV2 detected in several animal species from diverse countries. These findings confirmed that these two wild boars were infected by OvGHV2, represent the first description of this infection in these animals, and add to the number of pathogens identified in this animal species. Furthermore, these findings contrast earlier descriptions of OvGHV2 in swine since in all previous reports the infected pigs demonstrated clinical manifestations of disease. Consequently, these wild boars from Southern Brazil were subclinically infected or suffered asymptomatic infections by OvGHV2.