Meta-analytical review of antioxidant mechanisms responses in animals exposed to herbicide 2,4-D herbicide

• Published in: The Science of the total environment 2024-05, Vol.924, p.171680-171680, Article 171680
• Main Authors: da Silva, Ana Paula, Poquioma Hernández, Hilda Vanessa, Comelli, Camila Luiza, Guillén Portugal, Miguel Angel, Moreira Delavy, Fernanda, de Souza, Tugstênio Lima, de Oliveira, Elton Celton, de Oliveira-Ribeiro, Ciro Alberto, Silva de Assis, Helena Cristina, de Castilhos Ghisi, Nédia
• Format: Article
• Language: English
• Subjects: Catalase; Glutathione peroxidase; Glutathione reductase; Glutathione S-transferase; Pesticides; Superoxide dismutase
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.171680

The 2,4-Dichlorophenoxyacetic acid (2,4-D) is a low-cost herbicide to eradicate broadleaf weeds. Since the development of 2,4-D resistant transgenic crops, it has been described as one of the most widely distributed pollutants in the world, increasing concern about its environmental impacts. This study aimed to elucidate the antioxidant system response in animals exposed to 2,4-D by different routes of exposure. It focused on determining if tissue, phylogenetic group, and herbicide formulation would influence the antioxidant mechanisms. A careful literature search of Scopus, WoS, and Science Direct retrieved 6983, 24,098, and 20,616 articles, respectively. The dataset comprised 390 control-treatment comparisons and included three routes of exposure: transgenerational, oral, and topical. The data set for transgenerational and oral exposure revealed oxidative stress through a decrease in enzymatic activities and the level of molecules of the antioxidant system. In contrast, topical exposure increased the oxidative stress. Tissue-specific analyses revealed that the transgenerational effects reduced hepatic catalase (CAT) activity. Oral exposure caused a variety of effects, including increased CAT activity in the prostate and decreased activity in various tissues. Mammals predominate in the transgenerational and oral groups, showing a significantly reduced activity of the antioxidant system. In contrast, in the topical exposure, an increased activity of oxidative stress biomarkers was observed in fish, earthworms, and mollusks. The effects of the 2,4-D formulation on oxidative stress responses showed significant differences between pure and commercial formulations, with oral exposure resulting in decreased activity and topical exposure increasing responses. In summary, orally exposed animals exhibited a clear decrease in enzyme activities, transgenerational exposure elicited tissue-specific prompted biochemical reductions, and topical exposure induced increased responses, emphasizing the need for unbiased exploration of the effects of 2,4-D on biomarkers of oxidative stress while addressing publication bias in oral and topical datasets. [Display omitted] •Transgenerational exposure: mainly significant reduction, differences among tissues.•Mammals show decreased antioxidant activity; non-mammals exhibit increased responses•No difference between commercial or pure formulation•Clear difference between oral (+) or topic (−) exposure