Unraveling the ecological mechanisms of Aluminum on microbial community succession in epiphytic biofilms on Vallisneria natans leaves: Novel insights from microbial interactions

• Published in: Journal of hazardous materials 2024-05, Vol.469, p.133932, Article 133932
• Main Authors: Peng, Xue, Zhang, Xiaowen, Li, Zhuxi, Zhang, Shuxian, Zhang, Xinyi, Zhang, Haokun, Lin, Qingwei, Li, Xia, Zhang, Lu, Ge, Fangjie, Wu, Zhenbin, Liu, Biyun
• Format: Article
• Language: English
• Subjects: Aluminum; Aluminum - toxicity; Biofilms; Epiphytic biofilms; Hydrocharitaceae; Microbial Interactions; Microbiota; Plant Leaves; Vallisneria natans
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.133932

The extensive use of aluminum (Al) poses an escalating ecological risk to aquatic ecosystems. The epiphytic biofilm on submerged plant leaves plays a crucial role in the regulation nutrient cycling and energy flow within aquatic environments. Here, we conducted a mesocosm experiment aimed at elucidating the impact of different Al concentrations (0, 0.6, 1.2, 2.0 mg/L) on microbial communities in epiphytic biofilms on Vallisneria natans. At 1.2 mg/L, the highest biofilms thickness (101.94 µm) was observed. Al treatment at 2.0 mg/L significantly reduced bacterial diversity, while micro-eukaryotic diversity increased. Pseudomonadota and Bacteroidota decreased, whereas Cyanobacteriota increased at 1.2 mg/L and 2.0 mg/L. At 1.2 and 2.0 mg/L. Furthermore, Al at concentrations of 1.2 and 2.0 mg/L enhanced the bacterial network complexity, while micro-eukaryotic networks showed reduced complexity. An increase in positive correlations among microbial co-occurrence patterns from 49.51% (CK) to 57.05% (2.0 mg/L) was indicative of augmented microbial cooperation under Al stress. The shift in keystone taxa with increasing Al concentration pointed to alterations in the functional dynamics of microbial communities. Additionally, Al treatments induced antioxidant responses in V. natans, elevating leaf reactive oxygen species (ROS) content. This study highlights the critical need to control appropriate concentration Al concentrations to preserve microbial diversity, sustain ecological functions, and enhance lake remediation in aquatic ecosystems. [Display omitted] •Al stimulated filamentous Cyanobacteriota growth, increasing epiphytic biofilm thickness.•Al enhanced micro-eukaryotic Shannon diversity but inhibited bacteria diversity.•Al had a greater impact on bacterial community assembly than micro-eukaryotic community.•Al stress might disrupt microbial food webs through top-down control.