Characterization of bifidobacteria by random DNA amplification

RAPD conditions were optimized to generate reproducible banding patterns by testing primers, thermocyclers and overall reproducibility in repeat DNA analysis and separate DNA extractions. Five primers were chosen on the basis of band intensity and distribution (between 2 and 10 bands) which clearly...

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Bibliographic Details
Published in:International journal of food microbiology 1998-09, Vol.43 (3), p.185-193
Main Authors: Vincent, Daniel, Roy, Denis, Mondou, Francine, Déry, Claude
Format: Article
Language:English
Subjects:
Animals
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bifidobacteria
BIFIDOBACTERIUM
Bifidobacterium - classification
Bifidobacterium - genetics
Biological and medical sciences
Cattle
Cluster Analysis
DNA Primers
DNA, Bacterial - analysis
Electronic Data Processing
Electrophoresis, Agar Gel
Food industries
Food Microbiology
Fundamental and applied biological sciences. Psychology
Humans
IDENTIFICACION
IDENTIFICATION
Microbiology
Plasmids - chemistry
Probiotics - chemistry
Probiotics - classification
Random amplified polymorphic DNA
Random Amplified Polymorphic DNA Technique
RAPD
Rats
Reproducibility of Results
Species identification
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Summary:RAPD conditions were optimized to generate reproducible banding patterns by testing primers, thermocyclers and overall reproducibility in repeat DNA analysis and separate DNA extractions. Five primers were chosen on the basis of band intensity and distribution (between 2 and 10 bands) which clearly distinguished among strains of Bifidobacterium adolescentis, B. animalis, B. bifidum, B. breve, B. infantis and B. longum. The use of five single-primer reactions under optimized conditions improved the resolution and accuracy of the RAPD method for the characterization of dairy-related bifidobacteria. The results indicated that this method was highly reproducible in repeated analysis. Similarity between bifidobacteria strains was evaluated based on their RAPD profiles. Using a set of five primers, it was demonstrated that it may be possible to distinguish three different species of Bifidobacterium ( B. breve, B. bifidum and B. adolescentis), based on similarity of the RAPD profiles to known reference strains. Furthermore, application of the RAPD technique may also be useful and faster, than traditional systematics for placement of industrial strains into specific clusters (either B. longum/infantis or B. animalis/lactis).
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(98)00109-3