Phylogenomics and morphology of Celmisiinae (Asteraceae: Astereae): Taxonomic and evolutionary implications

• Published in: Molecular phylogenetics and evolution 2024-06, Vol.195, p.108064-108064, Article 108064
• Main Authors: Nicol, Duncan A., Saldivia, Patricio, Summerfield, Tina C., Heads, Michael, Lord, Janice M., Khaing, Ei P., Larcombe, Matthew J.
• Format: Article
• Language: English
• Subjects: Angiosperms353; Gene tree discordance; Hyb-Seq; Paragone-nf; Shawia; Target sequence capture
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2024.108064

[Display omitted] •First phylogenomic analysis of Celmisiinae.•Sixteen phylogenetic trees were estimated with paralogy management strategies.•Extensive morphological assessment of Celmisiinae.•Monophyly of three main clades identified despite high polyploidy and discordance.•Phylogenetic and morphological evidence help resolve the classification of Olearia. The tribe Astereae (Asteraceae) includes 36 subtribes and 252 genera, and is distributed worldwide in temperate and tropical regions. One of the subtribes, Celmisiinae Saldivia, has been recently circumscribed to include six genera and ca. 160 species, and is restricted to eastern Australia, New Zealand, and New Guinea. The species show an impressive range of growth habit, from small herbs and ericoid subshrubs to medium-sized trees. They live in a wide range of habitats and are often dominant in subalpine and alpine vegetation. Despite the well-supported circumscription of Celmisiinae, uncertainties have remained about their internal relationships and classification at genus and species levels. This study exploited recent advances in high-throughput sequencing to build a robust multi-gene phylogeny for the subtribe Celmisiinae. The target enrichment Angiosperms353 bait set and the hybpiper-nf and paragone-nf pipelines were used to retrieve, infer, and assemble orthologous loci from 75 taxa representing all the main putative clades within the subtribe. Because of the diploidised ploidy level in Celmisiinae, as well as missing data in the assemblies, uncertainty remains surrounding the inference of orthology detection. However, based on a variety of gene-family sets, coalescent and concatenation-based phylogenetic reconstructions recovered similar topologies. Paralogy and missing data in the gene-families caused some problems, but the estimated phylogenies were well-supported and well-resolved. The phylogenomic evidence supported Celmisiinae and three main clades: the Pleurophyllum clade (Pleurophyllum, Macrolearia and Damnamenia), mostly in the New Zealand Subantarctic Islands, Celmisia of mainland New Zealand and Australia, and Shawia (including ‘Olearia pro parte’ and Pachystegia) of New Zealand, Australia and New Guinea. The results presented here add to the accumulating support for the Angiosperms353 bait set as an efficient method for documenting plant diversity.