Using sulfate-amended sediment slurry batch reactors to evaluate mercury methylation

• Published in: Archives of environmental contamination and toxicology 2007-04, Vol.52 (3), p.326-331
• Main Authors: HARMON, S. M, KING, J. K, GLADDEN, J. B, NEWMAN, L. A
• Format: Article
• Language: English
• Subjects: Animal tissues; Animal, plant and microbial ecology; Applied ecology; Bacteria; Batch reactors; Bioaccumulation; Biological and medical sciences; Bioreactors; Colony Count, Microbial; Copper; Copper - analysis; Copper - metabolism; Ecotoxicology, biological effects of pollution; Fundamental and applied biological sciences. Psychology; General aspects; Geologic Sediments - microbiology; Mercury; Mercury - metabolism; Methylation; Methylmercury; Methylmercury Compounds - analysis; Methylmercury Compounds - metabolism; Public health; Reactors; Slurries; Slurry reactors; Sulfate reduction; Sulfates; Sulfates - pharmacology; Sulfur-Reducing Bacteria - drug effects; Sulfur-Reducing Bacteria - growth & development; Sulfur-Reducing Bacteria - metabolism; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - metabolism
• ISSN: 0090-4341; 1432-0703
• DOI: 10.1007/s00244-006-0071-x

In the methylated form, mercury represents a concern to public health primarily through the consumption of contaminated fish tissue. Research conducted on the methylation of mercury strongly suggests that the process is microbial in nature and facilitated principally by sulfate-reducing bacteria. This study addressed the potential for mercury methylation by varying sulfate treatments and wetland-based soil in microbial slurry reactors with available inorganic mercury. Under anoxic laboratory conditions conducive to the growth of naturally occurring sulfate-reducing bacteria in the soil, it was possible to evaluate how various sulfate additions influenced the methylation of inorganic mercury added to overlying water as well as the sequestration of dissolved copper. Treatments included sulfate amendments ranging from 25 to 500 mg/L (0.26 to 5.2 mM) above the soil's natural sulfate level. Mercury methylation in sulfate treatments did not exceed that of the nonamended control during a 35-day incubation period. However, increases in methylmercury concentration were linked to bacterial growth and sulfate reduction. A time lag in methylation in the highest treatment correlated with an equivalent lag in bacterial growth. The decrease in dissolved copper ranged from 72.7% in the control to 99.7% in the highest sulfate treatment. It was determined that experimental systems such as these can provide some useful information but that they also have severe limitations once sulfate is depleted or if sulfate is used in excess.