Tracking spoilage bacteria in commercial poultry processing and refrigerated storage of poultry carcasses

• Published in: International journal of food microbiology 2004-03, Vol.91 (2), p.155-165
• Main Authors: Hinton, Arthur, Cason, J.A., Ingram, Kimberly D.
• Format: Article
• Language: English
• Subjects: Acinetobacter; Aeromonas; Animals; Bacteria - classification; Bacteria - growth & development; Bacteria - isolation & purification; bacterial contamination; Biological and medical sciences; Brochothrix; broiler chickens; Broilers; chicken carcasses; Colony Count, Microbial; Cross-contamination; food contamination; Food Contamination - analysis; Food Handling - methods; Food industries; Food Microbiology; food pathogens; Food Preservation - methods; Food Preservation - standards; food processing equipment; food safety; food spoilage; food storage; Food-Processing Industry - methods; Food-Processing Industry - standards; Fundamental and applied biological sciences. Psychology; isolation; Meat and meat product industries; meat processing; microorganisms; monitoring; Myroides odoratus; pathogen identification; pathogen survival; Phylogeny; plate count; poultry industry; Poultry processing; Poultry Products - microbiology; psychrotrophic bacteria; quality control; Refrigeration; Shewanella putrefaciens; slaughterhouses; Spoilage bacteria; spoilage bacteria tracking; Time Factors; water
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/S0168-1605(03)00377-5

Four trials were conducted to examine the effect of commercial processing and refrigerated storage on spoilage bacteria in the native microflora of broiler carcasses. Prescalded, picked, eviscerated, and chilled carcasses were obtained from a commercial processing facility, and psychrotrophs in the bacterial flora were enumerated on Iron Agar, Pseudomonas Agar, and STAA Agar. The size of the population of spoilage bacteria on processed carcasses stored at 4 °C for 7, 10, or 14 days was also determined. Bacterial isolates were identified and dendrograms of the fatty acid profiles of the isolates were prepared to determine the degree of relatedness of the isolates. Findings indicated that although some processing steps increased the level of carcass contamination by selected bacteria, the number of spoilage bacteria recovered from processed carcasses was significantly ( P≤0.05) less than the number of bacteria recovered from carcasses entering the processing line. Acinetobacter and Aeromonas spp. were the primary isolates recovered from carcasses taken from the processing line. During refrigerated storage, there was a significant ( P≤0.05) increase in the population of bacteria on the carcasses, and Pseudomonas spp. were the predominant bacteria recovered from these carcasses. Dendrograms of the fatty acid profiles of the isolates indicated that bacterial cross-contamination of carcasses occurs during all stages of processing and that some bacteria can survive processing and proliferate on carcasses during refrigerated storage. Furthermore, cross-contamination was detected between carcasses processed on different days at the same facility. Findings indicate that although poultry processing decreases carcass contamination by psychrotrophic spoilage bacteria, significant levels of bacterial cross-contamination occur during processing, and bacteria that survive processing may multiply on the carcasses during refrigerated storage.