Fc engineered anti‐virus therapeutic human IgG1 expressed in plants with altered binding to the neonatal Fc receptor

• Published in: Biotechnology journal 2024-03, Vol.19 (3), p.e2300552-n/a
• Main Authors: Park, Sol‐Ah, Lee, Yoonji, Hwang, Hyunjoo, Lee, Jeong Hwan, Kang, Yang Joo, Kim, Yerin, Jin, Caiquan, An, Hyun Joo, Oh, Yoo Jin, Hinterdorfer, Peter, Kim, Eunhye, Choi, Sun, Ko, Kisung
• Format: Article
• Language: English
• Subjects: antibody; antiviral activity; Fc engineering; glycosylation; rabies virus; transgenic plant
• ISSN: 1860-6768; 1860-7314
• DOI: 10.1002/biot.202300552

Production of therapeutic monoclonal antibody (mAb) in transgenic plants has several advantages such as large‐scale production and the absence of pathogenic animal contaminants. However, mAb with high mannose (HM) type glycans has shown a faster clearance compared to antibodies produced in animal cells. The neonatal Fc receptor (FcRn) regulates the persistence of immunoglobulin G (IgG) by the FcRn‐mediated recycling pathway, which salvages IgG from lysosomal degradation within cells. In this study, Fc‐engineering of antirabies virus therapeutic mAb SO57 with the endoplasmic reticulum (ER)‐retention peptide signal (Lys‐Asp‐Glu‐Leu; KDEL) (mAbpK SO57) in plant cell was conducted to enhance its binding activity to human neonatal Fc receptor (hFcRn), consequently improve its serum half‐life. Enzyme‐linked immunosorbent assay (ELISA) and Surface plasmon resonance assay showed altered binding affinity of the Fc region of three different mAbpK SO57 variants [M252Y/S254T/T256E (MST), M428L/N434S (MN), H433K/N434F (HN)] to hFcRn compared to wild type (WT) of mAbpK SO57. Molecular modeling data visualized the structural alterations in these mAbpK SO57. All of the mAbpK SO57 variants had HM type glycan structures similar to the WT mAbpK SO57. In addition, the neutralizing activity of the three variants against the rabies virus CVS‐11 was effective as the WT mAbpK SO57. These results indicate that the binding affinity of mAbpK SO57 variants to hFcRn can be modified without alteration of N‐glycan structure and neutralization activity. Taken together, this study suggests that Fc‐engineering of antirabies virus mAb can be applied to enhance the efficacy of therapeutic mAbs in plant expression systems. Graphical and Lay Summary The recombinant antirabies human monoclonal (mAb) SO57, produced in transgenic plants, underwent Fc‐engineering [M252Y/S254T/T256E (MST), M428L/N434S (MN), H433K/N434F (HN)] to boost its hFcRn binding and serum half‐life. This approach shows promise for enhancing therapeutic mAbs in plant expression systems, potentially improving efficacy against rabies virus.