Investigation on characterization and transfection of a novel multi-polyplex gene delivery system

• Published in: Journal of applied polymer science 2007-10, Vol.106 (2), p.1028-1033
• Main Authors: Nie, Yu, Yuan, Wen-Min, Gong, Tao, Lu, Jiao, Fu, Yao, Zhang, Zhi-Rong
• Format: Article
• Language: English
• Subjects: Applied sciences; biocompatibility; Biological and medical sciences; drug-delivery systems; Exact sciences and technology; gene; General pharmacology; Medical sciences; Miscellaneous; nanoparticle; Natural polymers; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physicochemistry of polymers; transfection
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.26773

pDNA was condensed by polycationic peptide polylysine (PLL) to form a core, and then encapsulated in biodegradable monomethoxy (poly ethylene glycol)‐poly(lactide‐co‐glycolide)‐monomethoxy (poly ethylene glycol) (PELGE) to form core‐shell nanoparticles (NPs) as a novel multi‐polyplex gene delivery system—PPD(PELGE‐PLL‐DNA). NPs were prepared by a double emulsification‐solvent evaporation technique, using F68 (Pluronic F68, namely Poloxamer 188) as surfactant (not traditional stabilizer PVA), and characterized by morphology, particle size, zeta potential, nuclease, and sonication protection ability, as well as transfection efficiency. Results showed that PPD had a regular spherical shape, with an average diameter of 155 ± 2.97 nm and a zeta potential of −25.6 ± 1.35 mV. PPD could protect plasmid DNA from nuclease degradation and sonication during preparation, while the transfection efficiencies in HepG2 cells and Hela cells were much higher than that of NPs without PLL. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007