Potential contributions of keystone species to intestinal ecosystem in patients with Crohn's disease

Ravelling the central but poorly understood issue that potential contributions of keystone species to intestinal ecosystem functioning of patients with certain life-altering diseases including Crohn's disease (CD). In this study, a combination of 16S rRNA gene amplicon sequencing and amplicon-o...

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Published in:Journal of applied microbiology 2024-04, Vol.135 (4)
Main Authors: Peng, Yuzhu, Zhao, Qi, Chen, Shuijiao, Li, Zhenyu, An, Di, Zhang, Xian
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creator Peng, Yuzhu
Zhao, Qi
Chen, Shuijiao
Li, Zhenyu
An, Di
Zhang, Xian
description Ravelling the central but poorly understood issue that potential contributions of keystone species to intestinal ecosystem functioning of patients with certain life-altering diseases including Crohn's disease (CD). In this study, a combination of 16S rRNA gene amplicon sequencing and amplicon-oriented metagenomic profiling was applied to gain insights into the shifts in bacterial community composition at different stages of CD course, and explore the functional roles of identified keystone species in intestinal microecosystem. Our results showed significant alterations in structure and composition of gut microbiota between CD patients and healthy control (HC) (P 
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Metagenome-wide functional analyses further revealed that the relative abundance of specialized metabolism-related genes such as cynS, frdB, serA, and gltB from these bacterial species in CD group was significantly different (P &lt; 0.05) from that in HC, and highlighted the potential roles of the keystone species in regulating the accumulation of important metabolites such as succinate, formate, ammonia, L-glutamate, and L-serine, which might have an effect on homeostasis of intestinal ecosystem. 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title Potential contributions of keystone species to intestinal ecosystem in patients with Crohn's disease
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