Nα-acetyltransferase NAA50 mediates plant immunity independent of the Nα-acetyltransferase A complex

• Published in: Plant physiology (Bethesda) 2024-07, Vol.195 (4), p.3097-3118
• Main Authors: Armbruster, Laura, Pożoga, Marlena, Wu, Zhongshou, Eirich, Jürgen, Thulasi Devendrakumar, Karen, De La Torre, Carolina, Miklánková, Pavlina, Huber, Monika, Bradic, Fabian, Poschet, Gernot, Weidenhausen, Jonas, Merker, Sabine, Ruppert, Thomas, Sticht, Carsten, Sinning, Irmgard, Finkemeier, Iris, Li, Xin, Hell, Rüdiger, Wirtz, Markus
• Format: Article
• Language: English
• Subjects: Acetyltransferases - genetics; Acetyltransferases - metabolism; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - metabolism; Arabidopsis - microbiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Gene Expression Regulation, Plant; N-Terminal Acetyltransferase A - genetics; N-Terminal Acetyltransferase A - metabolism; N-Terminal Acetyltransferase E - genetics; N-Terminal Acetyltransferase E - metabolism; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Immunity - genetics; Pseudomonas syringae - pathogenicity; Pseudomonas syringae - physiology; Salicylic Acid - metabolism
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1093/plphys/kiae200

Abstract In humans and plants, 40% of the proteome is cotranslationally acetylated at the N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex is comprised of the catalytic subunit Nα-acetyltransferase 10 (NAA10) and the ribosome-anchoring subunit NAA15. The regulatory subunit Huntingtin Yeast Partner K (HYPK) and the acetyltransferase NAA50 join this complex in humans. Even though both are conserved in Arabidopsis (Arabidopsis thaliana), only AtHYPK is known to interact with AtNatA. Here we uncover the AtNAA50 interactome and provide evidence for the association of AtNAA50 with NatA at ribosomes. In agreement with the latter, a split-luciferase approach demonstrated close proximity of AtNAA50 and AtNatA in planta. Despite their interaction, AtNatA/HYPK and AtNAA50 exerted different functions in vivo. Unlike NatA/HYPK, AtNAA50 did not modulate drought tolerance or promote protein stability. Instead, transcriptome and proteome analyses of a novel AtNAA50-depleted mutant (amiNAA50) implied that AtNAA50 negatively regulates plant immunity. Indeed, amiNAA50 plants exhibited enhanced resistance to oomycetes and bacterial pathogens. In contrast to what was observed in NatA-depleted mutants, this resistance was independent of an accumulation of salicylic acid prior to pathogen exposure. Our study dissects the in vivo function of the NatA interactors HYPK and NAA50 and uncovers NatA-independent roles for NAA50 in plants. The Nα-acetyltransferase NAA50 interacts with the ribosome-tethered protein complex NatA to form the evolutionary conserved NatA/E complex but controls plant immunity independent of NatA function.