Functional analysis of basidiomycete specific chitin synthase genes in the agaricomycete fungus Pleurotus ostreatus

• Published in: Fungal genetics and biology 2024-06, Vol.172, p.103893-103893, Article 103893
• Main Authors: Schiphof, Kim, Kawauchi, Moriyuki, Tsuji, Kenya, Yoshimi, Akira, Tanaka, Chihiro, Nakazawa, Takehito, Honda, Yoichi
• Format: Article
• Language: English
• Subjects: Agaricomycete; Basidiomycete specific chitin synthase; Basidiomycota - enzymology; Basidiomycota - genetics; Cell wall; Cell Wall - genetics; Cell Wall - metabolism; Chitin; Chitin - metabolism; Chitin Synthase - genetics; Evolution, Molecular; Fungal Proteins - genetics; Fungal Proteins - metabolism; Phylogeny; Pleurotus - enzymology; Pleurotus - genetics; Pleurotus ostreatus
• ISSN: 1087-1845; 1096-0937
• DOI: 10.1016/j.fgb.2024.103893

•Three clades of basidiomycete specific chitin synthases were identified.•Disruption of chsb2–4 impacts hyphal morphology and cell wall thickness.•Δchsb2–4 strains are sensitive to cell wall and membrane, not oxidative stressors. Chitin is an essential structural component of fungal cell walls composed of transmembrane proteins called chitin synthases (CHSs), which have a large range of reported effects in ascomycetes; however, are poorly understood in agaricomycetes. In this study, evolutionary and molecular genetic analyses of chs genes were conducted using genomic information from nine ascomycete and six basidiomycete species. The results support the existence of seven previously classified chs clades and the discovery of three novel basidiomycete-specific clades (BI–BIII). The agaricomycete fungus Pleurotus ostreatus was observed to have nine putative chs genes, four of which were basidiomycete-specific. Three of these basidiomycete specific genes were disrupted in the P. ostreatus 20b strain (ku80 disruptant) through homologous recombination and transformants were obtained (Δchsb2, Δchsb3, and Δchsb4). Despite numerous transformations Δchsb1 was unobtainable, suggesting disruption of this gene causes a crucial negative effect in P. ostreatus. Disruption of these chsb2–4 genes caused sparser mycelia with rougher surfaces and shorter aerial hyphae. They also caused increased sensitivity to cell wall and membrane stress, thinner cell walls, and overexpression of other chitin and glucan synthases. These genes have distinct roles in the structural formation of aerial hyphae and cell walls, which are important for understanding basidiomycete evolution in filamentous fungi.