Presence of Trypanosoma cruzi TcI and Trypanosoma dionisii in sylvatic bats from Yucatan, Mexico

• Published in: Transactions of the Royal Society of Tropical Medicine and Hygiene 2024-10, Vol.118 (10), p.659-665
• Main Authors: Moo-Millan, Joel Israel, Tu, Weihong, de Jesús Montalvo-Balam, Teresa, Ibarra-López, Martha Pilar, Hernández-Betancourt, Silvia, Jesús May-Concha, Irving, Ibarra-Cerdeña, Carlos Napoleón, Barnabé, Christian, Dumonteil, Eric, Waleckx, Etienne
• Format: Article
• Language: English
• Subjects: Animals; Chagas Disease - epidemiology; Chagas Disease - transmission; Chagas Disease - veterinary; Chiroptera - parasitology; DNA, Protozoan; Mexico - epidemiology; Polymerase Chain Reaction; Prevalence; Rodentia - parasitology; Trypanosoma - classification; Trypanosoma - genetics; Trypanosoma - isolation & purification; Trypanosoma cruzi - genetics; Trypanosoma cruzi - isolation & purification
• ISSN: 0035-9203; 1878-3503; 1878-3503
• DOI: 10.1093/trstmh/trae023

Abstract Background Chagas disease is caused by Trypanosoma cruzi, whose genetic structure is divided into six discrete typing units (DTUs) known as TcI-TcVI. In the Yucatan Peninsula, Mexico, information regarding the DTUs circulating in wild mammals is scarce, while this is important knowledge for our understanding of T. cruzi transmission dynamics. Methods In the current study, we sampled wild mammals in a sylvatic site of the Yucatan Peninsula and assessed their infection with T. cruzi by PCR. Then, for infected mammals, we amplified and sequenced nuclear and mitochondrial T. cruzi genetic markers for DTU identification. Results In total, we captured 99 mammals belonging to the orders Chiroptera, Rodentia and Didelphimorphia. The prevalence of infection with T. cruzi was 9% (9/99; 95% CI [5, 16]), and we identified TcI in a Jamaican fruit bat, Artibeus jamaicensis. Moreover, we fortuitously identified Trypanosoma dionisii in another Jamaican fruit bat and detected an unidentified Trypanosoma species in a third specimen. While the latter discoveries were not expected because we used primers designed for T. cruzi, this study is the first to report the identification of T. dionisii in a bat from Yucatan, Mexico, adding to a recent first report of T. dionisii in bats from Veracruz, and first report of this Trypanosoma species in Mexico. Conclusion Further research is needed to enhance our knowledge of T. cruzi DTUs and Trypanosoma diversity circulating in wildlife in Southeastern Mexico.