SOX4 induces cytoskeleton remodeling and promotes cell motility via N-wasp/ARP2/3 pathway in colorectal cancer cells

• Published in: Experimental cell research 2024-06, Vol.439 (1), p.114059, Article 114059
• Main Authors: S, Anupriya, Parida, Nandita, Patnaik, Srinivas
• Format: Article
• Language: English
• Subjects: Colorectal cancer; Cytoskeleton remodeling; Filopodia; Invasion; Migration
• ISSN: 0014-4827; 1090-2422; 1090-2422
• DOI: 10.1016/j.yexcr.2024.114059

Filopodia are thin, actin-rich projection from the plasma membrane that promote cancer cell invasion and migration. Sex-determining region Y-related high-mobility group-box 4 (SOX4) is a crucial transcription factor that plays a role in the development and metastasis of colorectal cancer (CRC). However, the involvement of SOX4 in cytoskeleton remodeling in CRC remains unknown. For the first time, we demonstrate that SOX4 is a potent regulator of filopodia formation in CRC cells. Overexpression of SOX4 protein enhances both migration and invasion ability of HCT116, and CACO2 cells, which is relevant to the metastasis. Furthermore, through phalloidin staining, cytoskeleton re-assembly was observed in SOX4-modified cell lines. Enhanced expression of SOX4 increased the number and length of filopodia on cell surface. In contrast, silencing SOX4 in SW620 cells with higher endogenous expression of SOX4, impeded the filopodia formation. Moreover, SOX4 was found to be positively regulating the expression of central regulators of actin cytoskeleton - N-Wiskott-Aldrich syndrome protein (N-WASP); WAVE2; Actin related proteins, ARP2 and ARP3. Inhibiting the N-WASP/ARP2/3 pathway diminishes the filopodia formation and the migration of CRC cells. These results indicate the crucial role of SOX4 in the regulation of filopodia formation mediated by N-WASP/ARP2/3 pathway in CRC cells. [Display omitted] •Enhanced SOX4 expression boosts CRC cell migration and invasion via cytoskeleton remodeling.•SOX4 overexpression increases filopodia in HCT116 and CACO2 whereas silencing SOX4 reduces filopodia formation in SW620.•SOX4 mediates cytoskeleton rearrangement and migration/invasion through N-WASP/ARP2/3 pathway.