A biocompatible electrode/exoelectrogens interface augments bidirectional electron transfer and bioelectrochemical reactions

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2024-08, Vol.158, p.108723-108723, Article 108723
• Main Authors: Fang, Zhen, Hu, Jiani, Xu, Meng-Yuan, Li, Shan-Wei, Li, Chunmei, Zhou, Xiangtong, Wei, Jing
• Format: Article
• Language: English
• Subjects: Aniline Compounds - chemistry; Aniline Compounds - metabolism; Bidirectional electron transfer; Bio-denitrification; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; Bioelectric Energy Sources - microbiology; Bioelectrochemical system; Electroactive biofilm; Electrochemical Techniques - methods; Electrodes; Electron Transport; Graphite - chemistry; Nanocomplex electrode; Nanocomposites - chemistry; Shewanella - metabolism
• ISSN: 1567-5394; 1878-562X
• DOI: 10.1016/j.bioelechem.2024.108723

[Display omitted] •GO/PANI nanocomplex electrode with high biocompatibility was fabricated.•GO/PANI@CP electrode showed 54 times of power density compared to blank CP.•Bacteria on electrode surface showed fast electron release and uptake.•Efficient bio-denitrification in cathode was developed via GO/PANI@CP electrode. Bidirectional electron transfer is about that exoelectrogens produce bioelectricity via extracellular electron transfer at anode and drive cytoplasmic biochemical reactions via extracellular electron uptake at cathode. The key factor to determine above bioelectrochemical performances is the electron transfer efficiency under biocompatible abiotic/biotic interface. Here, a graphene/polyaniline (GO/PANI) nanocomposite electrode specially interfacing exoelectrogens (Shewanella loihica) and augmenting bidirectional electron transfer was conducted by in-situ electrochemical modification on carbon paper (CP). Impressively, the GO/PANI@CP electrode tremendously improved the performance of exoelectrogens at anode for wastewater treatment and bioelectricity generation (about 54 folds increase of power density compared to blank CP electrode). The bacteria on electrode surface not only showed fast electron release but also exhibited high electricity density of extracellular electron uptake through the proposed direct electron transfer pathway. Thus, the cathode applications of microbial electrosynthesis and bio-denitrification were developed via GO/PANI@CP electrode, which assisted the close contact between microbial outer-membrane cytochromes and nanocomposite electrode for efficient nitrate removal (0.333 mM/h). Overall, nanocomposite modified electrode with biocompatible interfaces has great potential to enhance bioelectrochemical reactions with exoelectrogens.