Comprehensive quality evaluation of Lysimachia christinae Hance via fingerprint, spectrum–effect relationship, and quantitative analyses of multiple components by single marker

• Published in: Phytochemical analysis 2024-08, Vol.35 (6), p.1527-1536
• Main Authors: LuoRong, Quji, Tan, Li‐Hong, Yu, Bao, Wu, Yingqin, Luo, Juan, Cao, Wei‐Guo, Li, Jingjing, Chen, Huan, Zhang, Dan
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - analysis; Anti-Inflammatory Agents - pharmacology; anti‐inflammatory; Calculi; Cholecystitis; choleretic; Chromatography, High Pressure Liquid - methods; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Error analysis; Female; fingerprint techniques; Fingerprints; Flavonoids; Flavonoids - analysis; Liquid chromatography; Lysimachia - chemistry; Lysimachia christinae; Lysimachia christinae Hance; Male; Mass spectrometry; Mass spectroscopy; Mice; Pharmacodynamics; Quadrupoles; Qualitative analysis; Quality assessment; Quality Control; quality evaluation; Rats; Rutin
• ISSN: 0958-0344; 1099-1565; 1099-1565
• DOI: 10.1002/pca.3394

Background Lysimachia christinae Hance (LCH) is a traditional medicine used to treat gallstone disease and cholecystitis. Despite its known anti‐inflammatory and choleretic effects, its quality has not been extensively evaluated. Objective In this study, we aimed to establish a reliable quality evaluation method for LCH via fingerprint, spectrum–effect relationship, and quantitative analyses of multicomponents by a single marker (QAMS). Methods First, the fingerprints and anti‐inflammatory and choleretic activities of 14 LCH batches were determined. Then, the gray relation analysis method was used to analyze the peak areas of the fingerprint profile and pharmacodynamic data. Subsequently, the characteristic peaks were tentatively identified using high‐performance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry. Finally, rutin was selected as the internal reference material, and QAMS was used to analyze the LCH components. Results Pharmacodynamic experiments confirmed that LCH exerted anti‐inflammatory and choleretic effects. Moreover, 15 flavonoids related to the anti‐inflammatory and choleretic effects of LCH were identified. Notably, relative error percentage between the QAMS and external standard method was less than 5%. Conclusion This study successfully established a comprehensive evaluation method for the qualitative and quantitative analyses of LCH. This article has two innovations: (1) The study confirmed the indicator chemical components that can reflect the anti‐inflammatory and choleretic effects of LCH and optimized the quality control indicators. (2) Based on the theory of QAMS, the study successfully established a rapid detection method for the indicator chemical components of LCH, effectively reducing inspection costs.