Tannic acid and quaternized chitosan mediated puerarin-loaded octacalcium phosphate /sodium alginate scaffold for bone tissue engineering

• Published in: International journal of biological macromolecules 2024-06, Vol.271 (Pt 2), p.132632, Article 132632
• Main Authors: Chen, Yan, Shi, Tengbin, Li, Lan, Hong, Ruchen, Lai, Jun, Huang, Tingting, Xu, Rui, Zhao, Qing, Chen, Xiaolong, Dai, Lijun, Zhou, Yuan, Liu, Wenge, Lin, Jinxin
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Animals; Anti-inflammatory; Bone and Bones - drug effects; Bone regeneration; Bone Regeneration - drug effects; Calcium Phosphates - chemistry; Calcium Phosphates - pharmacology; Cell Differentiation - drug effects; Chitosan - chemistry; Composite scaffolds; Humans; Isoflavones - chemistry; Isoflavones - pharmacology; Mice; Osteogenesis - drug effects; Polyphenols; Tannins - chemistry; Tannins - pharmacology; Tissue Engineering - methods; Tissue Scaffolds - chemistry
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132632

Current limitations in mechanical performance and foreign body reactions (FBR) often lead to implant failure, restricting the application of bioceramic scaffolds. This study presents a novel 3D-printed scaffold that combines the release of anti-inflammatory drugs with osteogenic stimulation. Initially, the inorganic and organic phases were integrated to ensure the scaffold's mechanical integrity through catechol chemistry and the electrostatic interactions between tannic acid and quaternary ammonium chitosan. Subsequently, layers of polydopamine-encapsulated puerarin-loaded zeolitic imidazolate framework-8 (ZIF-8) were self-assembled onto the stent's surface, creating the drug-loaded scaffold that improved drug release without altering the scaffold's structure. Compared with unloaded scaffolds, the puerarin-loaded scaffold demonstrated excellent osteogenic differentiation properties along with superior anti-inflammatory and osteogenic effects in a range of in vitro and in vivo studies. RNA sequencing clarified the role of the TNF and NF/κB signaling pathways in these effects, further supporting the scaffold's osteogenic potential. This study introduces a novel approach for creating drug-loaded scaffolds, providing a unique method for treating cancellous bone defects. [Display omitted]