Chondroitin sulfate from heads of corb: Recovery, structural analysis and assessment of anticoagulant activity

• Published in: Carbohydrate research 2024-07, Vol.541, p.109163-109163, Article 109163
• Main Authors: Bougatef, Hajer, Volpi, Nicola, Ben Amor, Ikram, Capitani, Federica, Maccari, Francesca, Gargouri, Jalel, Sila, Assaad, Bougatef, Ali
• Format: Article
• Language: English
• Subjects: Animals; Anticoagulant effect; Anticoagulants - chemistry; Anticoagulants - isolation & purification; Anticoagulants - pharmacology; Antiplatelet activity; Blood Coagulation - drug effects; Chondroitin sulfate; Chondroitin Sulfates - chemistry; Chondroitin Sulfates - isolation & purification; Chondroitin Sulfates - pharmacology; Constitutive disaccharides; Corb heads; Humans; Structural characterization
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2024.109163

In this study, glycosaminoglycans (GAGs) were extracted from corb (Sciaena umbra) heads and thoroughly examined for their structure. Through cellulose acetate electrophoresis, the GAGs were identified as chondroitin sulfate (CS), with a recovery yield of 10.35 %. The CS exhibited notable characteristics including a high sulfate content (12.4 %) and an average molecular weight of 38.32 kDa. Further analysis via 1H NMR spectroscopy and SAX-HPLC revealed that the CS primarily consisted of alternating units predominantly composed of monosulfated disaccharides at positions 6 and 4 of GalNAc (52.6 % and 38.8 %, respectively). The ratio of sulfate groups between positions 4 and 6 of GalNAc (4/6 ratio) was approximately 0.74, resulting in an overall charge density of 0.98. Thermal properties of the CS were assessed using techniques such as differential scanning calorimetry and thermogravimetric analysis. Notably, the CS demonstrated concentration-dependent prolongation of activated partial thromboplastin time (aPTT) and thrombin time (TT) while showing no effect on platelet function. At 200 μg/mL, aPTT and TT coagulation times were 1.4 and 3.7 times faster than the control, respectively. These findings suggest that CS derived from corb heads holds promise as an anticoagulant agent for therapy, although further clinical investigations are necessary to validate its efficacy. [Display omitted] •Chondroitin sulfate (CS) was purified from corb (Sciaena umbra) heads.•Structure and properties of CS were defined by SEM, SAX-HPLC, and 1H NMR.•Sulfation pattern and proportion of constitutive disaccharides were determined.•Monosulfated species ΔDi6s and ΔDi4s were found in the backbone.•Anticoagulant activity of CS was studied in vitro.