Effects of Dietary Melatonin on Antioxidant Capacity, Immune Defense, and Intestinal Microbiota in Red Swamp Crayfish (Procambarus clarkii)

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2024-08, Vol.26 (4), p.623-638
• Main Authors: Ye, Yucong, Huang, Jiarong, Li, Siwen, Li, Yiming, Zhao, Yunlong
• Format: Article
• Language: English
• Subjects: Abundance; Animal Feed - analysis; Animals; Antioxidants; Antioxidants - metabolism; Apoptosis; Apoptosis - drug effects; Aquaculture; Astacoidea - immunology; Astacoidea - microbiology; Biomedical and Life Sciences; Condition factor; Crayfish; Diet - veterinary; Dietary Supplements; Disease resistance; Engineering; Flora; Freshwater & Marine Ecology; Freshwater crustaceans; Gastrointestinal Microbiome - drug effects; Gene expression; Genes; Growth rate; Hepatopancreas; Hepatopancreas - metabolism; Immune response; Immune system; Intestinal flora; Intestinal microflora; Intestine; Lactobacilli; Life Sciences; Marine crustaceans; Melatonin; Melatonin - pharmacology; Microbiology; Microbiota; Microorganisms; Procambarus clarkii; Swamps; Zoology
• ISSN: 1436-2228; 1436-2236; 1436-2236
• DOI: 10.1007/s10126-024-10326-8

The aim of this study was to investigate the effects of melatonin (MT) feed supplementation on the antioxidant capacity, immune defense, and intestinal flora in Procambarus clarkii ( P. clarkii ). Six groups of P. clarkii were fed test feeds containing different levels of MT: 0 mg/kg (control), 22.5, 41.2, 82.7, 165.1, and 329.2 mg/kg for a duration of 2 months. The specific growth rate, hepatosomatic index, and condition factor were recorded highest in the test group of shrimp fed an MT concentration of 165.1 mg/kg. Compared to the control group, the rate of apoptosis was lower in hepatopancreas cells of P. clarkii supplemented with high concentrations of MT. Analyses of antioxidant capacity and immune-response-related enzymes in the hepatopancreas indicated that dietary supplementation of MT significantly augmented both the antioxidant system and immune responses. Dietary MT supplementation significantly increased the expression levels of antioxidant-immunity-related genes and decreased the expression levels of genes linked to apoptosis. Dietary MT was associated with an elevation in the abundance of the Firmicutes and a reduction in the abundance of the Proteobacteria in the intestines; besides, resulting in an increase in the abundance of beneficial bacteria, such as Lactobacilli . The broken-line model indicated that the suitable MT concentration was 154.09–157.09 mg/kg. MT supplementation enhanced the growth performance of P. clarkii , exerting a positive influence on the intestinal microbiota, and bolstered both immune response and disease resistance. Thus, this study offered novel perspectives regarding the application of dietary MT supplementation within the aquaculture field.