Stabilization of zein nanoparticles with tween-80 and fucoidan for encapsulation of eugenol via a nozzle simulation chip

• Published in: Food research international 2024-07, Vol.188, p.114514, Article 114514
• Main Authors: Lei, Yanlin, Lee, Youngsoo
• Format: Article
• Language: English
• Subjects: Antioxidant properties; Antioxidants - chemistry; Bioaccessibility; Delivery system; Drug Compounding; Essential oil; Eugenol - chemistry; Hydrogen-Ion Concentration; In vitro release; Molecular interaction; Nanoencapsulation; Nanoparticles - chemistry; Particle Size; Polysaccharides - chemistry; Polysorbates - chemistry; Zein - chemistry
• ISSN: 0963-9969; 1873-7145; 1873-7145
• DOI: 10.1016/j.foodres.2024.114514

[Display omitted] •Zein nanoparticle stability improved with tween-80 and fucoidan coating.•Eugenol encapsulated in zein-tween-80-fucoidan nanoparticles using a nozzle chip.•Encapsulation enhanced eugenol’s antioxidant and bioaccessibility properties in vitro.•Eugenol-loaded nanoparticles improved thermal and storage stability.•Nanoparticles have the potential for delivery of functional ingredients. Eugenol (EU), a natural bioactive compound found in various plants, offers numerous health benefits, but its application in the food and pharmaceutical industry is limited by its high volatility, instability, and low water solubility. Therefore, this study aimed to utilize the surface coating technique to develop zein-tween-80-fucoidan (Z-T-FD) composite nanoparticles for encapsulating eugenol using a nozzle simulation chip. The physicochemical characteristics of the composite nanoparticles were examined by varying the weight ratios of Z, T, and FD. Results showed that the Z-T-FD weight ratio of 5:1:15 exhibited excellent colloidal stability under a range of conditions, including pH (2–8), salt concentrations (10–500 mmol/L), heating (80 °C), and storage (30 days). Encapsulation of EU into Z-T-FD nanoparticles (0.5:5:1:15) resulted in an encapsulation efficiency of 49.29 ± 1.00%, loading capacity of 0.46 ± 0.05%, particle size of 205.01 ± 3.25 nm, PDI of 0.179 ± 0.006, and zeta-potential of 37.12 ± 1.87 mV. Spherical structures were formed through hydrophobic interaction and hydrogen bonding, as confirmed by Fourier transform infrared spectroscopy and molecular docking. Furthermore, the EU-Z-T-FD (0.5:5:1:15) nanoparticles displayed higher in vitro antioxidant properties (with DPPH and ABTS radical scavenging properties at 75.28 ± 0.16% and 39.13 ± 1.22%, respectively), in vitro bioaccessibility (64.78 ± 1.37%), and retention rates under thermal and storage conditions for EU compared to other formulations. These findings demonstrate that the Z-T-FD nanoparticle system can effectively encapsulate, protect, and deliver eugenol, making it a promising option for applications in the food and pharmaceutical industries.