A multi-step approach: Coupling of biodegradation and UV photocatalytic oxidation TiO2 for the treatment of naphthenic acid fraction compounds in oil sands process-affected water

• Published in: Chemosphere (Oxford) 2024-08, Vol.361, p.142502, Article 142502
• Main Authors: Miles, Sarah M., Balaberda, Amy-lynne, Leshuk, Timothy M.C., Peru, Kerry, Headley, John, Gu, Frank, Ulrich, Ania C.
• Format: Article
• Language: English
• Subjects: Bioremediation; Naphthenic acids; Oil sands; Oxidation; Photocatalytic
• ISSN: 0045-6535; 1879-1298; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.142502

Bitumen extraction in Alberta's oil sands region uses large volumes of water, leading to an abundance of oil sands process-affected water (OSPW). OSPW contains naphthenic acid fraction compounds (NAFCs) which have been found to contribute to OSPW toxicity. This study utilized a multistep treatment, coupling biological degradation with UV photocatalytic oxidation, and nutrient addition to boost the native microbial community's degradation capacity. OSPW initially contained 40–42 mg/L NAFCs with a toxicity of 3.8–3.9 TU. Initial biodegradation (Step 1) was used to remove the easily biodegradable NAFCs (11–25% removal), followed by a light or heavy dose of oxidation (Step 2) to breakdown the recalcitrant NAFCs (66–82% removal). Lastly, post-oxidation biodegradation with nutrients (Step 3) removed the residual bioavailable NAFCs (16–31% removal). By the end of the multistep treatment, the final NAFC concentrations and toxicity ranged from 5.3 to 6.8 mg/L and 1.1–1.2 TU. Analysis showed that OPSW was limited in phosphorus (below detection limit), and the addition of nutrients improved the degradation of NAFCs. Two treatments throughout the multistep treatment never received nutrients and showed minimal NAFC degradation post-oxidation. The native microbial community survived the stress from UV photocatalytic oxidation as seen by the post-oxidation NAFC biodegradation. Microbial community diversity was reduced considerably following oxidation, but increased with nutrient addition. The microbial community consisted predominately of Proteobacteria (Gammaproteobacteria and Alphaproteobacteria), and the composition shifted depending on the level of oxidation received. Possible NAFC-degrading microbes identified after a light oxidation dose included Pseudomonas, Acinetobacter and Xanthomonadales, while Xanthobacteracea and Rhodococcus were the dominant microbes after heavy oxidation. This experiment confirms that the microbial community is capable of degrading NAFCs and withstanding oxidative stress, and that degradation is further enhanced with the addition of nutrients. [Display omitted] •A multistep treatment was used: initial biodegradation, UV photocatalytic oxidation, and final biodegradation.•Significant NAFC removal and detoxification was observed.•Biodegradation was most effective with nutrient amendment.•Native microbes survived oxidation but diversity was significantly reduced.•Dominant microbial species shifted depending on the level of oxidation received.