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Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis
To explore the potential pathogenic genes of intestinal metaplasia. Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects underg...
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| Published in: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2024-05, Vol.44 (5), p.941 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | Chinese |
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| container_issue | 5 |
| container_start_page | 941 |
| container_title | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
| container_volume | 44 |
| creator | Pei, B Zhang, Y Wei, S Mei, Y Song, B Dong, G Wen, Z Li, X |
| description | To explore the potential pathogenic genes of intestinal metaplasia.
Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study. All the participants underwent gastroscopy and pathological examination, and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes (DEGs). The biological functions of the DEGs were analyzed using bioinformatics analysis, and qRT-PCR was used to validate the results.
Transcriptomic sequencing identified a total of 1373 DEGs, including 827 upregulated and 546 downregulated ones. The top 6 upregulated genes (
,
,
,
,
, and
), ranked based on their significance and average expression level, were selected for validation, and qRT-PCR showed significant upregulation of their mRNAs in |
| doi_str_mv | 10.12122/j.issn.1673-4254.2024.05.16 |
| format | article |
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Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study. All the participants underwent gastroscopy and pathological examination, and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes (DEGs). The biological functions of the DEGs were analyzed using bioinformatics analysis, and qRT-PCR was used to validate the results.
Transcriptomic sequencing identified a total of 1373 DEGs, including 827 upregulated and 546 downregulated ones. The top 6 upregulated genes (
,
,
,
,
, and
), ranked based on their significance and average expression level, were selected for validation, and qRT-PCR showed significant upregulation of their mRNAs in</description><identifier>ISSN: 1673-4254</identifier><identifier>DOI: 10.12122/j.issn.1673-4254.2024.05.16</identifier><identifier>PMID: 38862452</identifier><language>chi</language><publisher>China</publisher><subject>Computational Biology - methods ; Fatty Acid-Binding Proteins - genetics ; Female ; Gastric Mucosa - metabolism ; Gastric Mucosa - pathology ; Gene Expression Profiling ; Homeodomain Proteins - genetics ; Humans ; Intestines - pathology ; Male ; Metaplasia - genetics ; Mucin-2 - genetics ; Mucin-2 - metabolism ; RNA, Messenger - genetics ; Transcriptome</subject><ispartof>Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024-05, Vol.44 (5), p.941</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38862452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pei, B</creatorcontrib><creatorcontrib>Zhang, Y</creatorcontrib><creatorcontrib>Wei, S</creatorcontrib><creatorcontrib>Mei, Y</creatorcontrib><creatorcontrib>Song, B</creatorcontrib><creatorcontrib>Dong, G</creatorcontrib><creatorcontrib>Wen, Z</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><title>Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis</title><title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><description>To explore the potential pathogenic genes of intestinal metaplasia.
Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study. All the participants underwent gastroscopy and pathological examination, and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes (DEGs). The biological functions of the DEGs were analyzed using bioinformatics analysis, and qRT-PCR was used to validate the results.
Transcriptomic sequencing identified a total of 1373 DEGs, including 827 upregulated and 546 downregulated ones. The top 6 upregulated genes (
,
,
,
,
, and
), ranked based on their significance and average expression level, were selected for validation, and qRT-PCR showed significant upregulation of their mRNAs in</description><subject>Computational Biology - methods</subject><subject>Fatty Acid-Binding Proteins - genetics</subject><subject>Female</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gastric Mucosa - pathology</subject><subject>Gene Expression Profiling</subject><subject>Homeodomain Proteins - genetics</subject><subject>Humans</subject><subject>Intestines - pathology</subject><subject>Male</subject><subject>Metaplasia - genetics</subject><subject>Mucin-2 - genetics</subject><subject>Mucin-2 - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Transcriptome</subject><issn>1673-4254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNo9kD1PwzAQhj2AaFX6F1AGBpYEfyR2PKKKj0qVWGCOLoldjBI75NyhOz8cVxSWO-l537t7dYTcMlowzji__ywcoi-YVCIveVUWnPKyoFUiF2T5jxdkjehaWjGhaCXpFVmIupa8rPiSfG9746OzroPogs-CzaYQTwiGbIL4EfbGuy5L1eBJdT4ajM4neTQRpgHQQdYCmj5L83EGj93sphjGNIbm62B85_w-A99nrQvO2zCP6ViHCcFwRIfX5NLCgGZ97ivy_vT4tnnJd6_P283DLp8YlzFXWitIwa2pu7IyUDPQlmtdScVky2hbc85EXVqRDExq1ZZSa1Ero3oLQosVufvdO80h5cLYjA47MwzgTThgI6hUmrG6osl6c7Ye2tH0zTS7EeZj8_c58QMMBnVB</recordid><startdate>20240520</startdate><enddate>20240520</enddate><creator>Pei, B</creator><creator>Zhang, Y</creator><creator>Wei, S</creator><creator>Mei, Y</creator><creator>Song, B</creator><creator>Dong, G</creator><creator>Wen, Z</creator><creator>Li, X</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20240520</creationdate><title>Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis</title><author>Pei, B ; Zhang, Y ; Wei, S ; Mei, Y ; Song, B ; Dong, G ; Wen, Z ; Li, X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-7997a862fe8c45ea81a9f29956716b10b8221384f3e8c1697b4699387e7dfa393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2024</creationdate><topic>Computational Biology - methods</topic><topic>Fatty Acid-Binding Proteins - genetics</topic><topic>Female</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gastric Mucosa - pathology</topic><topic>Gene Expression Profiling</topic><topic>Homeodomain Proteins - genetics</topic><topic>Humans</topic><topic>Intestines - pathology</topic><topic>Male</topic><topic>Metaplasia - genetics</topic><topic>Mucin-2 - genetics</topic><topic>Mucin-2 - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Transcriptome</topic><toplevel>online_resources</toplevel><creatorcontrib>Pei, B</creatorcontrib><creatorcontrib>Zhang, Y</creatorcontrib><creatorcontrib>Wei, S</creatorcontrib><creatorcontrib>Mei, Y</creatorcontrib><creatorcontrib>Song, B</creatorcontrib><creatorcontrib>Dong, G</creatorcontrib><creatorcontrib>Wen, Z</creatorcontrib><creatorcontrib>Li, X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pei, B</au><au>Zhang, Y</au><au>Wei, S</au><au>Mei, Y</au><au>Song, B</au><au>Dong, G</au><au>Wen, Z</au><au>Li, X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis</atitle><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><date>2024-05-20</date><risdate>2024</risdate><volume>44</volume><issue>5</issue><spage>941</spage><pages>941-</pages><issn>1673-4254</issn><abstract>To explore the potential pathogenic genes of intestinal metaplasia.
Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study. All the participants underwent gastroscopy and pathological examination, and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes (DEGs). The biological functions of the DEGs were analyzed using bioinformatics analysis, and qRT-PCR was used to validate the results.
Transcriptomic sequencing identified a total of 1373 DEGs, including 827 upregulated and 546 downregulated ones. The top 6 upregulated genes (
,
,
,
,
, and
), ranked based on their significance and average expression level, were selected for validation, and qRT-PCR showed significant upregulation of their mRNAs in</abstract><cop>China</cop><pmid>38862452</pmid><doi>10.12122/j.issn.1673-4254.2024.05.16</doi></addata></record> |
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| identifier | ISSN: 1673-4254 |
| ispartof | Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024-05, Vol.44 (5), p.941 |
| issn | 1673-4254 |
| language | chi |
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| source | PubMed |
| subjects | Computational Biology - methods Fatty Acid-Binding Proteins - genetics Female Gastric Mucosa - metabolism Gastric Mucosa - pathology Gene Expression Profiling Homeodomain Proteins - genetics Humans Intestines - pathology Male Metaplasia - genetics Mucin-2 - genetics Mucin-2 - metabolism RNA, Messenger - genetics Transcriptome |
| title | Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis |
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