Sex differences in spontaneous respiratory recovery following chronic C2 hemisection

Respiratory deficits after C2 hemisection (C2Hx) have been well documented through single sex investigations. Although ovarian sex hormones enable enhanced respiratory recovery observed in females two weeks post-C2Hx, it remains unknown if sex impacts spontaneous respiratory recovery at chronic time...

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Bibliographic Details
Published in:Journal of applied physiology (1985) 2024-07, Vol.137 (1), p.166-180
Main Authors: Holmes, Taylor C, Popp, Nicole M, Hintz, Carley F, Dobrzycki, Isabell, Schmitz, Carolyn J, Schwichtenberg, Kaylyn A, Gonzalez-Rothi, Elisa J, Sundberg, Christopher W, Streeter, Kristi A
Format: Article
Language:English
Subjects:
Amplitudes
Animals
Atrophy
Data recovery
Diaphragm
Diaphragm (Anatomy)
Electromyography
Females
Fibers
Gender differences
Histology
Hormones
Longitudinal studies
Males
Muscles
Sex differences
Sex hormones
Sexes
Sleep and wakefulness
Spinal cord injuries
Therapeutic applications
Ventilation
Wakefulness
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Summary:Respiratory deficits after C2 hemisection (C2Hx) have been well documented through single sex investigations. Although ovarian sex hormones enable enhanced respiratory recovery observed in females two weeks post-C2Hx, it remains unknown if sex impacts spontaneous respiratory recovery at chronic time points. We conducted a longitudinal study to provide a comprehensive sex-based characterization of respiratory neuromuscular recovery for 8 weeks after C2Hx. We recorded ventilation and chronic diaphragm electromyography (EMG) output in awake behaving animals, phrenic motor output in anesthetized animals, and performed diaphragm muscle histology in chronically injured male and female rodents. Our results show that females expressed a greater recovery of tidal volume and minute ventilation compared to males during sub-acute and chronic time points. Eupneic diaphragm EMG amplitude during wakefulness and phrenic motor amplitude are similar between sexes at all time points after injury. Our data also suggests that females have a greater reduction in ipsilateral diaphragm EMG amplitude during spontaneous deep breaths (e.g., sighs) compared to males. Finally, we show evidence for atrophy and remodeling of the fast, fatigable fibers ipsilateral to injury in females, but not males. To our knowledge, the data presented here represent the first study to report sex-dependent differences in spontaneous respiratory recovery and diaphragm muscle morphology following chronic C2Hx. These data highlight the need to study both sexes to inform evidence-based therapeutic interventions in respiratory recovery post-SCI.
ISSN:8750-7587
1522-1601
1522-1601
DOI:10.1152/japplphysiol.00040.2024