Long-term exposure of metamifop affects sex differentiation and reproductive system of zebrafish (Danio rerio)

• Published in: Aquatic toxicology 2024-08, Vol.273, p.107004, Article 107004
• Main Authors: Guo, Mengyu, Zhao, Feng, Zhang, Mengna, Chen, Xiangguang, Duan, Manman, Xie, Yao, Zhang, Zhongyu, Jiang, Jiazhen, Qiu, Lihong
• Format: Article
• Language: English
• Subjects: Animals; Aromatase - genetics; Aromatase - metabolism; Endocrine disrupting effect; Estradiol; Female; Gonads - drug effects; Herbicides - toxicity; Male; Metamifop; Reproduction - drug effects; Sex differentiation; Sex Differentiation - drug effects; Testosterone; Transcription Factors - genetics; Transcription Factors - metabolism; Vitellogenins - genetics; Vitellogenins - metabolism; Water Pollutants, Chemical - toxicity; Zebrafish; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism
• ISSN: 0166-445X; 1879-1514; 1879-1514
• DOI: 10.1016/j.aquatox.2024.107004

•Metamifop affected the expression of sex differentiation-related genes.•Metamifop disrupted the balance of sex hormones in zebrafish.•Long-term exposure of metamifop caused feminization in zebrafish.•Metamifop may have potential endocrine disrupting effects. The extensive use of herbicide metamifop (MET) in rice fields for weeds control will inevitably lead to its entering into water environments and threaten the aquatic organisms. Previous researches have demonstrated that sublethal exposure of MET significantly affected zebrafish development. Yet the long-term toxicological impacts of MET on aquatic life remains unknown. Herein, we investigated the potential effects of MET (5 and 50 μg/L) on zebrafish during an entire life cycle. Since the expression level of male sex differentiation-related gene dmrt1 and sex hormone synthesis-related gene cyp19a1b were significantly changed after 50 μg/L MET exposure for only 7 days, indicators related to sex differentiation and reproductive system were further investigated. Results showed that the transcript of dmrt1 was inhibited, estradiol content increased and testosterone content decreased in zebrafish of both sexes after MET exposure at 45, 60 and 120 dpf. Histopathological sections showed that the proportions of mature germ cells in the gonads of male and female zebrafish (120 dpf) were significantly decreased. Moreover, males had elevated vitellogenin content while females did not after MET exposure; MET induced feminization in zebrafish, with the proportion of females significantly increased by 19.6% while that of males significantly decreased by 13.2% at 120 dpf. These results suggested that MET interfered with the expression levels of gonad development related-genes, disrupted sex hormone balance, and affected sex differentiation and reproductive system of female and male zebrafish, implying it might have potential endocrine disrupting effects after long-term exposure.