Single-cell topographical profiling of the immune synapse reveals a biomechanical signature of cytotoxicity

Immune cells have intensely physical lifestyles characterized by structural plasticity and force exertion. To investigate whether specific immune functions require stereotyped mechanical outputs, we used super-resolution traction force microscopy to compare the immune synapses formed by cytotoxic T...

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Published in:Science immunology 2024-06, Vol.9 (96), p.eadj2898
Main Authors: de Jesus, Miguel, Settle, Alexander H, Vorselen, Daan, Gaetjens, Thomas K, Galiano, Michael, Romin, Yevgeniy, Lee, Esther, Wong, Yung Yu, Fu, Tian-Ming, Santosa, Endi, Winer, Benjamin Y, Tamzalit, Fella, Wang, Mitchell S, Santella, Anthony, Bao, Zhirong, Sun, Joseph C, Shah, Pavak, Theriot, Julie A, Abel, Steven M, Huse, Morgan
Format: Article
Language:English
Subjects:
Animals
Biomechanical Phenomena - immunology
Cytotoxicity, Immunologic
Immunological Synapses - immunology
Macrophages - immunology
Mice
Mice, Inbred C57BL
Single-Cell Analysis
T-Lymphocytes, Cytotoxic - immunology
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Summary:Immune cells have intensely physical lifestyles characterized by structural plasticity and force exertion. To investigate whether specific immune functions require stereotyped mechanical outputs, we used super-resolution traction force microscopy to compare the immune synapses formed by cytotoxic T cells with contacts formed by other T cell subsets and by macrophages. T cell synapses were globally compressive, which was fundamentally different from the pulling and pinching associated with macrophage phagocytosis. Spectral decomposition of force exertion patterns from each cell type linked cytotoxicity to compressive strength, local protrusiveness, and the induction of complex, asymmetric topography. These features were validated as cytotoxic drivers by genetic disruption of cytoskeletal regulators, live imaging of synaptic secretion, and in silico analysis of interfacial distortion. Synapse architecture and force exertion were sensitive to target stiffness and size, suggesting that the mechanical potentiation of killing is biophysically adaptive. We conclude that cellular cytotoxicity and, by implication, other effector responses are supported by specialized patterns of efferent force.
ISSN:2470-9468
2470-9468
DOI:10.1126/sciimmunol.adj2898