Prolonged exposure to hypergravity increases number and size of cells and enhances lignin deposition in the stem of Arabidopsis thaliana

• Published in: Journal of plant research 2024-09, Vol.137 (5), p.927-937
• Main Authors: Shinohara, Hironori, Muramoto, Masaki, Tamaoki, Daisuke, Kamachi, Hiroyuki, Inoue, Hiroshi, Kume, Atsushi, Karahara, Ichirou
• Format: Article
• Language: English
• Subjects: Acceleration; Arabidopsis - growth & development; Arabidopsis - physiology; Arabidopsis thaliana; Biomedical and Life Sciences; Cell number; Cell size; Deposition; Exposure; Fibers; Gravitational effects; Gravity; High gravity environments; Hypergravity; Life Sciences; Lignin; Lignin - metabolism; Long-term effects; Physical characteristics; Plant Biochemistry; Plant Ecology; Plant Leaves - growth & development; Plant Physiology; Plant Sciences; Plant Stems - anatomy & histology; Plant Stems - growth & development; Plants (botany); Regular Paper – Morphology/Anatomy/Structural Biology; Rosette; Stems; Xylem
• ISSN: 0918-9440; 1618-0860; 1618-0860
• DOI: 10.1007/s10265-024-01556-x

We have performed a lab-based hypergravity cultivation experiment using a centrifuge equipped with a lighting system and examined long-term effects of hypergravity on the development of the main axis of the Arabidopsis ( Arabidopsis thaliana (L.) Heynh.) primary inflorescence, which comprises the rachis and peduncle, collectively referred to as the main stem for simplicity. Plants grown under 1 × g (gravitational acceleration on Earth) conditions for 20–23 days and having the first visible flower bud were exposed to hypergravity at 8 × g for 10 days. We analyzed the effect of prolonged hypergravity conditions on growth, lignin deposition, and tissue anatomy of the main stem. As a result, the length of the main stem decreased and cross-sectional area, dry mass per unit length, cell number, and lignin content of the main stem significantly increased under hypergravity. Lignin content in the rosette leaves also increased when they were exposed to hypergravity during their development. Except for interfascicular fibers, cross-sectional areas of the tissues composing the internode significantly increased under hypergravity in most types of the tissues in the basal part than the apical part of the main stem, indicating that the effect of hypergravity is more pronounced in the basal part than the apical part. The number of cells in the fascicular cambium and xylem significantly increased under hypergravity both in the apical and basal internodes of the main stem, indicating a possibility that hypergravity stimulates procambium activity to produce xylem element more than phloem element. The main stem was suggested to be strengthened through changes in its morphological characteristics as well as lignin deposition under prolonged hypergravity conditions.