Multiple-readout lateral flow immunoassay for the sensitive detection of nitrofurazone metabolites through ultrabright AIE-MOF coupled in-situ growth strategy

• Published in: Biosensors & bioelectronics 2024-10, Vol.262, p.116556, Article 116556
• Main Authors: Cheng, Yuanyuan, Yin, Xuechi, Li, Yuechun, Wang, Shaochi, Xue, Shouyu, Wu, Qiaoying, Wang, Jianlong, Zhang, Daohong
• Format: Article
• Language: English
• Subjects: Aggregation-induced emission; Metal-organic frameworks; Nitrofurazone metabolites; Prussian blue nanoparticles; Triple-readout lateral flow immunoassay
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2024.116556

The multiple-readout capability of multimodal detection enhances the flexibility, reliability, and accuracy of lateral flow immunoassay (LFIA). The conjugation of two different metal-organic frameworks (MOFs) as a new-generation composite material offers extraordinary opportunities for developing multimodal LFIA. It is anticipated to compensate limitations of traditional single colorimetric signal LFIA and improve the analysis performance. Herein, an ultra-bright fluorescent AIE-MOF was proposed and coupled with an in-situ growth of Prussian blue (PB) nanoparticles strategy to obtain a novel multimodal signal tracer (AIE-MOF@PB). Thereafter, it was successfully applied to develop the multimodal LFIA platform for the detection of nitrofurazone metabolites. The synergy of AIE-MOF and PB endows AIE-MOF@PB with superb water dispersibility, robust fluorescence emission, brilliant colorimetric signal, marvelous photothermal conversion, and enhanced antibody coupling efficiency, all of which facilitate a highly sensitive triple-readout LFIA platform. The detection sensitivity improved by at least 5-fold compared with the colloidal gold-based LFIA. This work not only inspires the rational design of aggregation-induced emission luminogens (AIEgen)-based complex materials but also highlights the promising potential in flexible point-of-care applications. •AIE-MOF with boosted fluorescent emission was fabricated.•PB modification increases biocompatibility and water dispersibility.•Synergy endows tracer with fluorescence, colorimetric, and photothermal signals.•Triple-readout LFIA improves detection performance and flexibility.•Compared to AuNP-LFIA, assay sensitivity is improved by 17 times (FL mode).