Cold tolerance of woodland strawberry (Fragaria vesca) is linked to Cold Box Factor 4 and the dehydrin Xero2

Abstract Domesticated strawberry is susceptible to sudden frost episodes, limiting the productivity of this cash crop in regions where they are grown during early spring. In contrast, the ancestral woodland strawberry (Fragaria vesca) has successfully colonized many habitats of the Northern Hemisphe...

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Published in:Journal of experimental botany 2024-09, Vol.75 (18), p.5857-5879
Main Authors: Kanbar, Adnan, Weinert, Christoph Hubertus, Kottutz, David, Thinh, La, Abuslima, Eman, Kabil, Farida, Hazman, Mohamed, Egert, Björn, Trierweiler, Bernhard, Kulling, Sabine Emma, Nick, Peter
Format: Article
Language:English
Subjects:
Acclimatization
Cold Temperature
Fragaria - genetics
Fragaria - growth & development
Fragaria - metabolism
Fragaria - physiology
Gene Expression Regulation, Plant
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana - physiology
Plant Proteins - genetics
Plant Proteins - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Abstract Domesticated strawberry is susceptible to sudden frost episodes, limiting the productivity of this cash crop in regions where they are grown during early spring. In contrast, the ancestral woodland strawberry (Fragaria vesca) has successfully colonized many habitats of the Northern Hemisphere. Thus, this species seems to harbour genetic factors promoting cold tolerance. Screening a germplasm established in the frame of the German Gene Bank for Crop Wild Relatives, we identified, among 70 wild accessions, a pair with contrasting cold tolerance. By following the physiological, biochemical, molecular, and metabolic responses of this contrasting pair, we identified the transcription factor Cold Box Factor 4 and the dehydrin Xero2 as molecular markers associated with superior tolerance to cold stress. Overexpression of green fluorescent protein fusions with Xero2 in tobacco BY-2 cells conferred cold tolerance to these recipient cells. A detailed analysis of the metabolome for the two contrasting genotypes allows the definition of metabolic signatures correlated with cold tolerance versus cold stress. This work provides a proof-of-concept for the value of crop wild relatives as genetic resources to identify genetic factors suitable to increase the stress resilience of crop plants. Two woodland strawberry accessions contrasting in cold tolerance were characterized for their responses to cold stress, and Cold Box Factor 4 and Xero2 were identified as predictors for cold tolerance. Graphical Abstract Graphical Abstract
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/erae263