How lignin biosynthesis responds to nitrogen in plants: a scoping review

• Published in: Plant biology (Stuttgart, Germany) Germany), 2024-10, Vol.26 (6), p.881-895
• Main Authors: Peng, Q., Shrestha, A., Zhang, Y., Fan, J., Yu, F., Wang, G.
• Format: Article
• Language: English
• Subjects: Amino acids; Biosynthesis; Biosynthetic Pathways; Carbohydrates; Gene Expression Regulation, Plant; genes; Lignin; Lignin - biosynthesis; Lignin - metabolism; Metabolites; Nitrogen; Nitrogen - metabolism; Phenols; Phenylpropanoids; Plant hormones; Plant layout; Plant species; Plants - metabolism; Reviews; Secondary metabolites; Transcription factors
• ISSN: 1435-8603; 1438-8677; 1438-8677
• DOI: 10.1111/plb.13627

Nitrogen (N) plays a critical role in the functioning of key amino acids and synthetic enzymes responsible for the various stages of lignin biosynthesis. However, the precise mechanisms through which N influences lignin biosynthesis have not been fully elucidated. This scoping review explores how lignin biosynthesis responds to N in plants. A systematic search of the literature in several databases was conducted using relevant keywords. Only 44 of the 1842 selected studies contained a range of plant species, experimental conditions, and research approaches. Lignin content, structure, and biosynthetic pathways in response to N are discussed, and possible response mechanisms of lignin under low N are proposed. Among the selected studies, 64.52% of the studies reter to lignin content found a negative correlation between N availability and lignin content. Usually, high N decreases the lignin content, delays cell lignification, increases p‐hydroxyphenyl propane (H) monomer content, and regulates lignin synthesis through the expression of key genes (PAL, 4CL, CCR, CAD, COMT, LAC, and POD) encoding miRNAs and transcription factors (e.g., MYB, bHLH). N deficiency enhances lignin synthesis through the accumulation of phenylpropanoids, phenolics, and soluble carbohydrates, and indirect changes in phytohormones, secondary metabolites, etc. This review provides new insights and important references for future studies on the regulation of lignin biosynthesis. High Nitrogen decreases lignin content, delays cell lignification, increases p‐hydroxyphenyl propane monomer content, and regulates lignin synthesis through expression of some key genes encoding miRNAs and transcription factors; N deficiency enhances lignin synthesis through accumulation of phenylpropanoids, phenolics, and soluble carbohydrates, and indieect changes in phytohormones, secondary metabolites, etc.