Synthesis of hydrogels from biomaterials and their potential application in tissue engineering

• Published in: Carbohydrate research 2024-09, Vol.543, p.109216, Article 109216
• Main Authors: Martínez-Mejía, Gabriela, Cuadras-Arconada, Ricardo, Vázquez-Torres, Nadia Adriana, Caro-Briones, Rubén, Castell-Rodríguez, Andrés, del Río, José Manuel, Corea, Mónica, Jiménez-Juárez, Rogelio
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - chemical synthesis; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Chitosan - chemical synthesis; Chitosan - chemistry; Chitosan - pharmacology; Cross-Linking Reagents - chemistry; Glutaral - chemistry; Hydrogels - chemical synthesis; Hydrogels - chemistry; Rheology; Tissue Engineering
• ISSN: 0008-6215; 1873-426X; 1873-426X
• DOI: 10.1016/j.carres.2024.109216

In this study, a series of hydrogels were synthesized from chitosan(s) that was crosslinking with glutaraldehyde at different concentrations. Ascorbic acid in an acidic medium was used to facilitate non-covalent interactions. The chitosan(s) was obtained from shrimp cytoskeleton; while ascorbic acid was extracted from xoconostle juice. The hydrogel reaction was monitored by UV–vis spectroscopy (550 nm) to determine the reaction kinetics and reaction order at 60 °C. The hydrogels structures were characterized by NMR, FT-IR, HR-MS and SEM, while the degree of cross-linking was examined with TGA-DA. The extracellular matrices were obtained as stable hydrogels where reached maximum crosslinking was of 7 %, independent of glutaraldehyde quantity added. The rheological properties showed a behavior of weak gels and a dependence of crosslinking agent concentration on strength at different temperatures. The cytotoxicity assay showed that the gels had no adverse effects on cellular growth for all concentrations of glutaraldehyde. [Display omitted]