The structure of the γ‐TuRC at the microtubule minus end – not just one solution

• Published in: BioEssays 2024-09, Vol.46 (9), p.e2400117-n/a
• Main Authors: Gao, Qi, Vermeulen, Bram J. A., Würtz, Martin, Shin, Hyesu, Erdogdu, Dilara, Zheng, Anjun, Hofer, Florian W., Neuner, Annett, Pfeffer, Stefan, Schiebel, Elmar
• Format: Article
• Language: English
• Subjects: Animals; Assembly; centrosome; CM1 motif; cryo‐electron microscopy; Humans; microtubule minus end; Microtubule-Associated Proteins - chemistry; Microtubule-Associated Proteins - metabolism; Microtubules; Microtubules - metabolism; Molecular structure; Nucleation; spindle‐pole body; Tubulin; Tubulin - chemistry; Tubulin - metabolism; Vertebrates; γ‐tubulin complexes
• ISSN: 0265-9247; 1521-1878; 1521-1878
• DOI: 10.1002/bies.202400117

In cells, microtubules (MTs) assemble from α/β‐tubulin subunits at nucleation sites containing the γ‐tubulin ring complex (γ‐TuRC). Within the γ‐TuRC, exposed γ‐tubulin molecules act as templates for MT assembly by interacting with α/β‐tubulin. The vertebrate γ‐TuRC is scaffolded by γ‐tubulin‐interacting proteins GCP2‐6 arranged in a specific order. Interestingly, the γ‐tubulin molecules in the γ‐TuRC deviate from the cylindrical geometry of MTs, raising the question of how the γ‐TuRC structure changes during MT nucleation. Recent studies on the structure of the vertebrate γ‐TuRC attached to the end of MTs came to varying conclusions. In vitro assembly of MTs, facilitated by an α‐tubulin mutant, resulted in a closed, cylindrical γ‐TuRC showing canonical interactions between all γ‐tubulin molecules and α/β‐tubulin subunits. Conversely, native MTs formed in a frog extract were capped by a partially closed γ‐TuRC, with some γ‐tubulin molecules failing to align with α/β‐tubulin. This review discusses these outcomes, along with the broader implications. Recent work using cryo‐electron microscopy has shed a lot of light on the mechanism of microtubule (MT) nucleation and capping by its essential nucleator, the γ‐tubulin ring complex. In this review, we summarize the advancements in our understanding of MT nucleation and identify their implications from a structural perspective.