Transcriptomics-based analysis of Macrobrachium rosenbergii growth retardation

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Genomics & proteomics, 2024-12, Vol.52, p.101298, Article 101298
• Main Authors: Li, Xuenan, Li, Yahui, Dai, Xilin
• Format: Article
• Language: English
• Subjects: Animals; Differentially expressed genes; Gene Expression Profiling; Gills - growth & development; Gills - metabolism; Growth retardation; Hepatopancreas - metabolism; Macrobrachium rosenbergii; Palaemonidae - genetics; Palaemonidae - growth & development; Transcriptome; Transcriptome analysis
• ISSN: 1744-117X; 1878-0407; 1878-0407
• DOI: 10.1016/j.cbd.2024.101298

Macrobrachium rosenbergii is an economically important crustacean in many parts of the world, but in recent years, growth retardation has become an increasingly serious issue. While the underlying causes remain unclear, this has inevitably impacted on aquaculture and production outputs. In this study, gill, hepatopancreas, and muscle tissue samples from M. rosenbergii, with distinct growth differences, underwent transcriptome sequencing and bioinformatics analyses using high-throughput sequencing. In total, 59,796 unigenes were annotated. Differential expression analyses showed that the most differentially expressed genes (DEGs) were screened in gill tissue (1790 DEGs). In muscle and hepatopancreas tissues, 696 and 598 DEGs were screened, respectively. These DEGs were annotated to Kyoto Encyclopedia of Genes and Genomes pathways, which identified several significantly enriched pathways related to growth metabolism, such as PI3K-AKT, glycolysis/gluconeogenesis, and starch and sucrose metabolism. These results suggest that low growth metabolism levels may be one cause of M. rosenbergii growth retardation. Our data provide support for further investigations into the causes and molecular mechanisms underpinning growth retardation in M. rosenbergii. [Display omitted] •In this study, transcriptome sequencing and bioinformatics analysis were performed on three tissues with growth differences.•Changes in intracellular homeostasis and ribosomal stress responses may also contribute to growth retardation in shrimp.•Low expression of growth metabolism genes may result in blocked energy synthesis and protein synthesis in vivo.