Acute and sub-acute Toxicity Profiles of Anchusa strigosa and Zataria Multiflora: Insights from wistar albino rats

• Published in: Toxicon (Oxford) 2024-10, Vol.249, p.108051, Article 108051
• Main Authors: Sheikh, Wajid Mohammad, Jadhav, Indrani, Shafi, Majid, Muzamil Bashir, Showkeen, Jan, Jasmeena, Kalam, Mohd Afsahul, Shah, Showkat Ahmad, Shah, Abdul Jalil, Yousuf, Shahid
• Format: Article
• Language: English
• Subjects: Acute toxicity; Biochemical analysis; Haematological parameters; Histopathological examination; Subacute toxicity
• ISSN: 0041-0101; 1879-3150; 1879-3150
• DOI: 10.1016/j.toxicon.2024.108051

The present study aims to assess the acute and subacute toxicity of the hydro-alcoholic extracts of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss in Wistar albino rats. The crude extracts of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss were prepared in 70% ethanol. Systematic tests for acute toxicity were performed at varying dosages of 100, 250, and 500 mg/kg, while for subacute toxicity, a dose of 600 mg/kg was orally given to Wistar albino rats. At the end of acute and sub-acute toxicity studies, biochemical parameters, hematological analysis, and histopathological analysis showed no significant difference in the body weight, abnormalities, or organ damage of the rats compared to the untreated rats (control). Also, there were no results of death recorded in rats. These findings indicated that the medium-term oral administration of Anchusa strigosa (leaves) and the aerial parts of Zataria multiflora Boiss after the treatment does not cause toxicity and provides assurance regarding their suitability for potential therapeutic applications in both acute and subacute forms. [Display omitted] •No evidence of acute and sub-acute toxicity for Anchusa strigosa and Zataria multiflora supports their short-term use in biomedical applications.•No aberrant observations were found in the biochemical, hematological, and histopathological analyses.•500 mg/kg is an optimum dosage of both plant extracts for therapeutic use.•Elevated liver markers indicate potential risks for long-term use.