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Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3
Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of Lactiplant...
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| Published in: | Current microbiology 2024-10, Vol.81 (10), p.303, Article 303 |
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| creator | Chai, Yunmei Ma, Qingwen He, Jinze Wei, Guangqiang Huang, Aixiang |
| description | Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of
Lactiplantibacillus plantarum
(
L. plantarum
) L3 was sequenced and characterized, and then linked the
L. plantarum L3
genome to the STRING database for QS system regulatory target prediction. A total of 3,167,484 base pairs (bps) were examined from the genome of
L. plantarum
L3, and 30 QS-related genes were discovered (including
luxS
). The STRING database prediction indicated that the 30 QS-related genes are mainly involved in the regulation of nine metabolic pathways. Furthermore,
metE
,
metK
,
aroB
,
cysE
, and
birA1
were predicted to be regulatory targets of the LuxS/AI-2 QS system, and these five targets were validated based on quantitative real-time PCR and content determination. Successful elucidation of the LuxS/AI-2 QS system’s key targets and regulation mechanism in
L. plantarum
L3 demonstrated the effectiveness of the new approach for predicting QS targets and provides a scientific basis for future work on improving regulation of functional factor production. |
| doi_str_mv | 10.1007/s00284-024-03834-2 |
| format | article |
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Lactiplantibacillus plantarum
(
L. plantarum
) L3 was sequenced and characterized, and then linked the
L. plantarum L3
genome to the STRING database for QS system regulatory target prediction. A total of 3,167,484 base pairs (bps) were examined from the genome of
L. plantarum
L3, and 30 QS-related genes were discovered (including
luxS
). The STRING database prediction indicated that the 30 QS-related genes are mainly involved in the regulation of nine metabolic pathways. Furthermore,
metE
,
metK
,
aroB
,
cysE
, and
birA1
were predicted to be regulatory targets of the LuxS/AI-2 QS system, and these five targets were validated based on quantitative real-time PCR and content determination. Successful elucidation of the LuxS/AI-2 QS system’s key targets and regulation mechanism in
L. plantarum
L3 demonstrated the effectiveness of the new approach for predicting QS targets and provides a scientific basis for future work on improving regulation of functional factor production.</description><identifier>ISSN: 0343-8651</identifier><identifier>ISSN: 1432-0991</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-024-03834-2</identifier><identifier>PMID: 39127970</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biofilms ; Biofilms - growth & development ; Biomedical and Life Sciences ; Biotechnology ; Carbon-Sulfur Lyases ; Gene Expression Regulation, Bacterial ; Gene regulation ; Genes ; Genome, Bacterial ; Genomes ; Lactiplantibacillus plantarum ; Lactobacillus plantarum - genetics ; Lactobacillus plantarum - metabolism ; Lactobacillus plantarum - physiology ; Life Sciences ; LuxS protein ; Lysine ; Lysine - metabolism ; Metabolic pathways ; Microbiology ; Original Paper ; Predictions ; Quorum Sensing ; Real time ; Regulation ; Strings</subject><ispartof>Current microbiology, 2024-10, Vol.81 (10), p.303, Article 303</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-6323d0d4618231fbef07e2ff09b60b33c537088065c6dc5730f090e5aa98c6cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39127970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chai, Yunmei</creatorcontrib><creatorcontrib>Ma, Qingwen</creatorcontrib><creatorcontrib>He, Jinze</creatorcontrib><creatorcontrib>Wei, Guangqiang</creatorcontrib><creatorcontrib>Huang, Aixiang</creatorcontrib><title>Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of
Lactiplantibacillus plantarum
(
L. plantarum
) L3 was sequenced and characterized, and then linked the
L. plantarum L3
genome to the STRING database for QS system regulatory target prediction. A total of 3,167,484 base pairs (bps) were examined from the genome of
L. plantarum
L3, and 30 QS-related genes were discovered (including
luxS
). The STRING database prediction indicated that the 30 QS-related genes are mainly involved in the regulation of nine metabolic pathways. Furthermore,
metE
,
metK
,
aroB
,
cysE
, and
birA1
were predicted to be regulatory targets of the LuxS/AI-2 QS system, and these five targets were validated based on quantitative real-time PCR and content determination. Successful elucidation of the LuxS/AI-2 QS system’s key targets and regulation mechanism in
L. plantarum
L3 demonstrated the effectiveness of the new approach for predicting QS targets and provides a scientific basis for future work on improving regulation of functional factor production.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbon-Sulfur Lyases</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Lactiplantibacillus plantarum</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Lactobacillus plantarum - metabolism</subject><subject>Lactobacillus plantarum - physiology</subject><subject>Life Sciences</subject><subject>LuxS protein</subject><subject>Lysine</subject><subject>Lysine - metabolism</subject><subject>Metabolic pathways</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Predictions</subject><subject>Quorum Sensing</subject><subject>Real time</subject><subject>Regulation</subject><subject>Strings</subject><issn>0343-8651</issn><issn>1432-0991</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU1P3DAQhi1EVba0f4ADssSFSk079iROcgQEFClSy0LPluNMVkb5WOwEiX-Pd5cWiUMPlmXPM69Hfhg7EvBdAOQ_AoAs0gRkXFhgmsg9thApygTKUuyzBWCKSaEyccA-hfAAIGQJ4iM7wFLIvMxhwaalWbuGL-mJTOeGFR9bfjuPfu75HQ1hc3N6e_c1WdJq7sxEDf9dnX3j525sXddzMzS8eo4Y8XvjVzSFTUBl7OTWnRkmVxvrum4OfHs0m9wKP7MPrekCfXndD9mfq8v7i59J9ev65uKsSqzM1JQolNhAkypRSBRtTS3kJNsWylpBjWgzzKEoQGVWNTbLEWIJKDOmLKyyDR6y013u2o-PM4VJ9y5Y6uIoNM5BI8R_KHKVioievEMfxtkPcbotlSJijpGSO8r6MQRPrV571xv_rAXojRO9c6KjE711omVsOn6Nnuuemn8tfyVEAHdAiKVhRf7t7f_EvgDasZVN</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Chai, Yunmei</creator><creator>Ma, Qingwen</creator><creator>He, Jinze</creator><creator>Wei, Guangqiang</creator><creator>Huang, Aixiang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20241001</creationdate><title>Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3</title><author>Chai, Yunmei ; Ma, Qingwen ; He, Jinze ; Wei, Guangqiang ; Huang, Aixiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-6323d0d4618231fbef07e2ff09b60b33c537088065c6dc5730f090e5aa98c6cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Carbon-Sulfur Lyases</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Lactiplantibacillus plantarum</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - metabolism</topic><topic>Lactobacillus plantarum - physiology</topic><topic>Life Sciences</topic><topic>LuxS protein</topic><topic>Lysine</topic><topic>Lysine - metabolism</topic><topic>Metabolic pathways</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Predictions</topic><topic>Quorum Sensing</topic><topic>Real time</topic><topic>Regulation</topic><topic>Strings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chai, Yunmei</creatorcontrib><creatorcontrib>Ma, Qingwen</creatorcontrib><creatorcontrib>He, Jinze</creatorcontrib><creatorcontrib>Wei, Guangqiang</creatorcontrib><creatorcontrib>Huang, Aixiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chai, Yunmei</au><au>Ma, Qingwen</au><au>He, Jinze</au><au>Wei, Guangqiang</au><au>Huang, Aixiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>81</volume><issue>10</issue><spage>303</spage><pages>303-</pages><artnum>303</artnum><issn>0343-8651</issn><issn>1432-0991</issn><eissn>1432-0991</eissn><abstract>Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of
Lactiplantibacillus plantarum
(
L. plantarum
) L3 was sequenced and characterized, and then linked the
L. plantarum L3
genome to the STRING database for QS system regulatory target prediction. A total of 3,167,484 base pairs (bps) were examined from the genome of
L. plantarum
L3, and 30 QS-related genes were discovered (including
luxS
). The STRING database prediction indicated that the 30 QS-related genes are mainly involved in the regulation of nine metabolic pathways. Furthermore,
metE
,
metK
,
aroB
,
cysE
, and
birA1
were predicted to be regulatory targets of the LuxS/AI-2 QS system, and these five targets were validated based on quantitative real-time PCR and content determination. Successful elucidation of the LuxS/AI-2 QS system’s key targets and regulation mechanism in
L. plantarum
L3 demonstrated the effectiveness of the new approach for predicting QS targets and provides a scientific basis for future work on improving regulation of functional factor production.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>39127970</pmid><doi>10.1007/s00284-024-03834-2</doi></addata></record> |
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| source | Springer Nature |
| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism Biofilms Biofilms - growth & development Biomedical and Life Sciences Biotechnology Carbon-Sulfur Lyases Gene Expression Regulation, Bacterial Gene regulation Genes Genome, Bacterial Genomes Lactiplantibacillus plantarum Lactobacillus plantarum - genetics Lactobacillus plantarum - metabolism Lactobacillus plantarum - physiology Life Sciences LuxS protein Lysine Lysine - metabolism Metabolic pathways Microbiology Original Paper Predictions Quorum Sensing Real time Regulation Strings |
| title | Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3 |
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