Lysosome-Targeted Polarity-Sensitive NIR Fluorescence Probe for Imaging Injured Lung and Liver in Diabetes

• Published in: Analytical chemistry (Washington) 2024-08, Vol.96 (34), p.14053
• Main Authors: Zhang, Jinzheng, Gong, Shengyi, Li, Qianhua, Zhang, Shiya, Feng, Guoqiang
• Format: Article
• Language: English
• Subjects: Animal models; Cell culture; Damage detection; Diabetes; Diabetes mellitus; Diagnosis; Fluorescent indicators; Hepatocytes; Hyperglycemia; Injuries; Liver; Liver diseases; Lung diseases; Lungs; Lysosomes; Near infrared radiation; Polarity; Probes; Target detection
• ISSN: 0003-2700; 1520-6882; 1520-6882
• DOI: 10.1021/acs.analchem.4c03214

Diabetes is a chronic disease marked by high blood glucose. With the progress of diabetes, complications gradually appear, and various organs may be affected. However, due to the lack of noninvasive in situ detection probes, the diagnosis of organ damage caused by diabetes is significantly delayed, which will cause many complications that cannot be treated in time. Here, we report a BODIPY-based fluorescent probe , which can be used to detect lung and liver damage caused by diabetes. By introducing methylpiperazine and extending the conjugated system, can locate lysosomes and exhibit absorption and emission both in the near-infrared (NIR) region. In addition, is sensitive to polarity and can be used for sensitive detection of lysosomal polarity changes. Unexpectedly, targets and images the lungs and liver of mice. Subsequently, hyperglycemia-stimulated cell models and diabetic mouse models were successfully established, and was utilized to reveal that polarity can be used as a diagnostic signal of diabetic complications. Notably, for the first time confirmed the lung injury and liver injury caused by diabetes using the fluorescent probes method, providing a new approach for the diagnosis of diabetes complications.