Development of poly(lactic acid)-based natural antimicrobial film incorporated with caprylic acid against Salmonella biofilm contamination in the meat industry

• Published in: International journal of food microbiology 2024-12, Vol.425, p.110871, Article 110871
• Main Authors: Her, Eun, Han, Sangha, Ha, Sang-Do
• Format: Article
• Language: English
• Subjects: Active packaging; Animals; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - pharmacology; ATR-FTIR; Beef; Biofilms - drug effects; Biofilms - growth & development; CA-PLA film; Caprylates - pharmacology; Cattle; Chicken breast; Chickens; FESEM; Food Contamination - prevention & control; Food Microbiology; Food Packaging - methods; Lactic Acid - pharmacology; Meat - microbiology; Microbial Sensitivity Tests; Polyesters - chemistry; Polyesters - pharmacology; Polymers - chemistry; Polymers - pharmacology; Salmonella - drug effects; Salmonella - growth & development; Salmonella enteritidis - drug effects; Salmonella enteritidis - growth & development; Salmonella typhimurium - drug effects; Salmonella typhimurium - growth & development
• ISSN: 0168-1605; 1879-3460; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2024.110871

Using a solvent-casting method, a poly(lactic acid) (PLA) film incorporated with caprylic acid (CA) was developed as an active packaging against Salmonella enterica ser. Typhimurium and S. enteritidis to reduce the risk of microbial contamination during distribution and storage of meat. According to the minimum inhibitory concentration (MIC) test results of the natural antimicrobial, CA was introduced at 0.6, 1.2, 2.4, and 4.8 % (v/v) into neat PLA. The biofilm inhibitory effect and antimicrobial efficacy of CA-PLA film against both Salmonella strains, as well as the intermolecular interactions and barrier properties of CA-PLA film, were evaluated. Biofilm formation was reduced to below the detection limit ( 0.05) changes were observed for water vapor permeability and oxygen permeability by the addition of CA into PLA film, suggesting that CA-PLA film is a promising strategy for active packaging to control Salmonella contamination in the meat industry. •The CA-PLA film maintained its typical chemical structure.•The CA-PLA performed antibiofilm activity against Salmonella contamination on meat product.•The CA-PLA film did not show any adverse effect on the color and texture qualities.•The CA-PLA film was effective to reduce virulence and stress-related, and biofilm-forming-related gene expression.