Interplay of co-cultured chimeric adipose and gingival tissues exacerbates inflammatory dysfunction relevant to periodontal and metabolic conditions

• Published in: Life sciences (1973) 2024-10, Vol.355, p.123009, Article 123009
• Main Authors: Xiao, Li, Mochizuki, Mai, Shimamura, Naohiro, Sunada, Katsuhisa, Nakahara, Taka
• Format: Article
• Language: English
• Subjects: Adipose Tissue - metabolism; Animals; Coculture Techniques; Collagen; Cytokines - metabolism; Gingiva - metabolism; Gingiva - pathology; Humans; Inflammation; Inflammation - metabolism; Inflammation - pathology; Lipid; Lipopolysaccharides; Macrophages - metabolism; Male; Metabolic syndrome; Metabolic Syndrome - metabolism; Mice; Mice, Inbred C57BL; Periodontitis; Periodontitis - metabolism; Periodontitis - pathology; Tissue engineering
• ISSN: 0024-3205; 1879-0631; 1879-0631
• DOI: 10.1016/j.lfs.2024.123009

Adipose tissue dysfunction is a key feature of metabolic syndrome, which increases the risk of periodontitis, an inflammatory disease induced by bacteria that affects the gingiva and other components of periodontal tissue. Recent studies indicate that molecules from inflamed periodontal tissue contribute to adipose tissue dysfunction. However, the cellular mechanisms and interactions between adipose tissue and gingiva driving the progression of metabolic and periodontal conditions remain unclear. To address this, we developed a chimeric (mouse/human) co-culture tissue model (which identifies the origins of species-specific cytokines) to investigate these interactions. Using tissue-specific functional cells and immunocytes, we constructed equivalents of adipose tissue (ATE) and gingiva (GTE), co-cultivating them under inflammatory conditions induced by bacterial endotoxin, lipopolysaccharide (LPS). Our findings showed that exposure to LPS resulted in a notable reduction in lipid accumulation, GLUT4 expression, and adiponectin secretion in ATE, along with increased macrophage colonies forming around lipid droplets, as well as elevated levels of triglyceride, leptin, and IL-6. In GTE, LPS triggered significant inflammatory responses, characterized by increased macrophage accumulation, elevated COX-2 expression, and heightened secretion of inflammatory cytokines. LPS also reduced epithelial thickness and the expression of keratin 19 and collagen IV, indicating impaired barrier function and gingival integrity. Co-culturing ATE with GTE exacerbated these LPS-induced harmful effects in both tissues. In conclusion, our findings suggest that interplay between gingiva and adipose tissue can intensify the inflammatory and dysfunctional changes caused by LPS. This co-culture tissue model offers a valuable tool for future studies on periodontitis and metabolic syndrome. •LPS impairs adipose tissue function and insulin sensitivity.•LPS disrupts gingival tissue barrier function and integrity.•LPS triggers inflammatory responses in adipose and gingival tissues.•Gingival and adipose tissues reciprocally exacerbate LPS-induced dysfunction.•Chimeric co-culture model identifies origins of species-specific cytokines.