Metabolic profile changes of zebrafish larvae in the single- and co-exposures of microplastics and phenanthrene

• Published in: The Science of the total environment 2024-11, Vol.953, p.175994, Article 175994
• Main Authors: Li, Jingyi, Liu, Xiao, Fu, Jing, Gong, Zhiyuan, Jiang, Sabrina Yanan, Chen, J. Paul
• Format: Article
• Language: English
• Subjects: Combined toxicity; Metabolomics; Microplastics; Phenanthrene; Zebrafish
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.175994

Microplastics (MPs) are ubiquitous in the environment, and can adsorb organic contaminants (OCs) and be taken by various microorganisms and organisms, which could eventually lead to risk to humans. In this study, the phenotypic changes and metabolic profile alternations of zebrafish in the single- and co-exposure of MPs and phenanthrene (Phe) were investigated. The results showed that significantly higher tail malformation rate and edema rate in zebrafish induced by MPs can be enhanced due to the co-existence of Phe. The metabolomic analysis revealed that both synergistic and antagonistic effects of MPs and Phe on the metabolic alternation of zebrafish larvae exist, since unique perturbations of metabolites or pathways were found in all of the three exposure scenarios. Based on Partial least squares-discriminant analysis, porphine, ribose, and L-glutamic acid were the most important metabolites resulting in the difference between the treated and control groups in the MP exposure, Phe exposure and co-exposure, respectively. Two dysregulated pathways namely d-glutamine and D-glutamate metabolism, and alanine, aspartate and glutamate metabolism were significantly affected in the co-exposure while not in either of the single exposure. These findings provide new insights into the toxic effects of MPs on aquatic organisms, and further studies on combined effects of MPs and OCs are suggested to be conducted. [Display omitted] •Microplastics and phenanthrene have both synergistic and antagonistic toxicological effects to zebrafish.•Phenanthrene enhanced the elevated edema rate and tail malformation rate induced by microplastics.•Perturbation patterns of metabolites and pathways were different in the single- and co-exposure.•Two metabolic pathways of amino acids were only affected under co-exposure.