Enhancing cell activities through integration of polyanionic alginate or hyaluronic acid derivatives with triboelectric nanogenerators

• Published in: Carbohydrate polymers 2024-12, Vol.346, p.122629, Article 122629
• Main Authors: Shakibi, Reyhaneh, Khayamian, Mohammad Ali, Abadijoo, Hamed, Dashtianeh, Mahshid, Kolahdouz, Mohammadreza, Daemi, Hamed, Abdolmaleki, Parviz
• Format: Article
• Language: English
• Subjects: Alginates - chemistry; Animals; Cell Movement - drug effects; Cell Proliferation - drug effects; Chitosan - chemistry; Electric Stimulation; Electrical stimulation; Fibroblasts - cytology; Fibroblasts - drug effects; Humans; Hyaluronic Acid - chemistry; Hyaluronic Acid - pharmacology; Hydrogel; Hydrogels - chemistry; Hydrogels - pharmacology; Mice; NIH 3T3 Cells; Polyelectrolytes - chemistry; Polysaccharide; Triboelectric nanogenerator; Wound healing; Wound Healing - drug effects
• ISSN: 0144-8617; 1879-1344; 1879-1344
• DOI: 10.1016/j.carbpol.2024.122629

The impact of electrical stimulation has been widely investigated on the wound healing process; however, its practicality is still challenging. This study explores the effect of electrical stimulation on fibroblasts in a culture medium containing different electrically-charged polysaccharide derivatives including alginate, hyaluronate, and chitosan derivatives. For this aim, an electrical stimulation, provided by a zigzag triboelectric nanogenerator (TENG), was exerted on fibroblasts in the presence of polysaccharides' solutions. The analyses showed a significant increase in cell proliferation and an improvement in wound closure (160 % and 90 %, respectively) for the hyaluronate-containing medium by a potential of 3 V after 48 h. In the next step, a photo-crosslinkable hydrogel was prepared based on hyaluronic acid methacrylate (HAMA). Then, the cells were cultured on HAMA hydrogel and treated by an electrical stimulation. Surprisingly, the results showed a remarkable increase in cell growth (280 %) and migration (82 %) after 24 h. Attributed to the electroosmosis phenomenon and an amplified transfer of soluble growth factors, a dramatic promotion was underscored in cell activities. These findings highlight the role of electroosmosis in wound healing, where TENG-based electrical stimulation is combined with bioactive polysaccharide-based hydrogels to promote wound healing. [Display omitted]