A simple culture medium for phenotypic characterization and long-term storage of medically relevant fusarioid fungi

Fusarioid fungi, particularly Neocosmospora solani and Fusarium oxysporum, are emerging as significant human pathogens, causing infections ranging from localized mycoses to life-threatening systemic diseases. Accurate identification and preservation of these fungi in clinical laboratories remain cha...

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Bibliographic Details
Published in:Journal of microbiological methods 2024-11, Vol.226, p.107042, Article 107042
Main Authors: Monteiro, Ruan Campos, Yu, Maria Cecília Zorat, Dolatabadi, Somayeh, de Aguiar Cordeiro, Rossana, Milanez, Edlâny Pinho Romão, Gonçalves, Sarah Santos, de Camargo, Zoilo Pires, Höfling-Lima, Ana Luisa, Rodrigues, Anderson Messias
Format: Article
Language:English
Subjects:
Bisifusarium
Conidia production
Culture media
Fusarium
Milk-honey and malt agar
Neocosmospora
Preservation
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Summary:Fusarioid fungi, particularly Neocosmospora solani and Fusarium oxysporum, are emerging as significant human pathogens, causing infections ranging from localized mycoses to life-threatening systemic diseases. Accurate identification and preservation of these fungi in clinical laboratories remain challenging because of their diverse morphologies and specific growth requirements. This study evaluated a novel milk-honey and malt agar (MHM) against conventional media for cultivating and preserving 60 clinical fusarioid isolates, including Neocosmospora spp. (n = 47), Bisifusarium spp. (n = 5), and Fusarium spp. (n = 8). Compared with Sabouraud dextrose 2 % agar (SDA) and malt extract agar (ME2), MHM significantly increased conidia production (p 
ISSN:0167-7012
1872-8359
1872-8359
DOI:10.1016/j.mimet.2024.107042