Harnessing 3D printed highly porous Ti-6Al-4V scaffolds coated with graphene oxide to promote osteogenesis

Bone tissue engineering (BTE) strategies have been developed to address challenges in orthopedic and dental therapy by expediting osseointegration and new bone formation. In this study, we developed irregular porous Ti-6Al-4V scaffolds coated with reduced graphene oxide (rGO), specifically rGO-pTi,...

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Published in:Biomaterials science 2024-10, Vol.12 (21), p.5491-553
Main Authors: Jang, Hee Jeong, Kang, Moon Sung, Jang, Jinju, Lim, Dohyung, Choi, Seong-Won, Jung, Tae-Gon, Chun, Heoung-Jae, Kim, Bongju, Han, Dong-Wook
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Graphene
Hemorrhage
In vivo methods and tests
Orthopedics
Protein adsorption
Regeneration (physiology)
Scaffolds
Tissue engineering
Titanium base alloys
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Summary:Bone tissue engineering (BTE) strategies have been developed to address challenges in orthopedic and dental therapy by expediting osseointegration and new bone formation. In this study, we developed irregular porous Ti-6Al-4V scaffolds coated with reduced graphene oxide (rGO), specifically rGO-pTi, and investigated their ability to stimulate osseointegration in vivo . The rGO-pTi scaffolds exhibited unique irregular micropores and high hydrophilicity, facilitating protein adsorption and cell growth. In vitro assays revealed that the rGO-pTi scaffolds increased alkaline phosphatase (ALP) activity, mineralization nodule formation, and osteogenic gene upregulation in MC3T3-E1 preosteoblasts. Moreover, in vivo transplantation of rGO-pTi scaffolds in rabbit calvarial bone defects showed improved bone matrix formation and osseointegration without hemorrhage. These findings highlight the potential of combining rGO with irregular micropores as a promising BTE scaffold for bone regeneration. Bone tissue engineering (BTE) strategies have been developed to address challenges in orthopedic and dental therapy by expediting osseointegration and new bone formation.
ISSN:2047-4830
2047-4849
2047-4849
DOI:10.1039/d4bm00970c