Isolates of Acanthamoeba species in the marine environment in the Philippines

spp. are considered the most commonly occurring free-living amoebae (FLA) in the environment. Their high resilience enables them to thrive in different types of environments. Using purposive sampling, 80 surface water samples were collected from identified coastal sites in Mariveles, Bataan, and Lin...

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Published in:Journal of water and health 2024-09, Vol.22 (9), p.1695-1703
Main Authors: Layson, Samantha Nicole, Alcala, Cheilo Maurrice D, Avenido, Mikael Lorenzo Q, Bayot, Aleeza Erika M, Aclan, Charles Darwin C, Barlis, Joepher S, Villacorta, Katrina D, Abalos, Venice Marielle R, Maramba, Alyssa Nicole M, Say, Maricel D C, Serrano, Alessandrea A, Cabello, Jana Katryn D, Salvosa, Julienne Marie I, Tan, Moriset Paz Djezla C, Uy, Kyla Franchesca F, Masangkay, Frederick Ramirez, Milanez, Giovanni De Jesus
Format: Article
Language:English
Subjects:
Acanthamoeba
Acanthamoeba - classification
Acanthamoeba - genetics
Acanthamoeba - isolation & purification
Aquaculture
Coastal waters
Genotypes
Human settlements
Infections
Marine environment
Nucleotide sequence
Pathogens
PCR
Philippines
Phylogeny
Polymerase chain reaction
Protozoa
Public health
Risk factors
Seawater - parasitology
Settling
Shorelines
Soil settlement
Surface water
Waste disposal
Water analysis
Water sampling
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Summary:spp. are considered the most commonly occurring free-living amoebae (FLA) in the environment. Their high resilience enables them to thrive in different types of environments. Using purposive sampling, 80 surface water samples were collected from identified coastal sites in Mariveles, Bataan, and Lingayen Gulf (40 water samples for each). Nineteen (23.75%) of the 80 water samples yielded positive amoebic growth during the 14-day culture and microscopic examination. The polymerase chain reaction confirmed spp. DNA in isolates MB1, A3, A4, A7, C5, and D3 using JDP1 and JDP2 primer sets. Further sequencing revealed that the isolates belonged to sp., , , and genotype T4. The sequences were deposited in GenBank and registered under accession numbers PP741651, PP767364, PP741728, PP741729, PP767365, and PP767366, respectively. Potential risk factors such as waste disposal, expansion of human settlements to coastal locations, and soil runoffs in these environments should be controlled to mitigate the proliferation of potentially pathogenic strains of FLAs.
ISSN:1477-8920
1996-7829
DOI:10.2166/wh.2024.163